Сytogenetical bioindication of pesticidal contamination

Cherednichenko, Oksana; Pilyugina, Anastassiya; Nuraliev, Serikbai

doi:10.1016/b978-0-323-90489-6.00010-0

Cited by 4 publications

(2 citation statements)

References 56 publications

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“…A cell population's proliferative state is gauged by the mitotic index. Mitotic index values in the present study may be lower than previously reported since fewer cells were found to be arrested in metaphase (Cherednichenko et al, 2022). From the results of the above experiment; the pre-exposure to Ginko Biloba at 100 mg/ kg.BW showed a protective effect against DNA damage that was represented by the significant decrease in the mitotic index in the group exposed previously G. Biloba extract.…”

Section: Groupscontrasting

confidence: 68%

Ginkgo Biloba Extract’s Efficacy to Mitigate the Genotoxicity that Hydroxyurea Induces in Mice

Al-Ameedi¹,

Ayad²,

Mohammed³

et al. 2023

AAVS

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Ginkgo Biloba is a potential medicinal plant used traditionally to treat various diseases. The present study aimed to evaluate the protective effect of Ginkgo Biloba against Genotoxicity Induced by Hyroxyurea in Mice. Forty male albino mice were randomly divided into 4 groups of ten animals. The first group received hydroxyurea (80mg/kg B. W) orally daily for 30 days, while second and third group received Ginkgo Biloba (100 mg/ kg.BW) and omega3 (150 mg/kg.BW) orally daily for two weeks of HU administration as protective and 4 th group (C-ve) were considered as control negative group was given distilled water orally. After therapy by removing the bone marrow from the animal's bone after anesthesia with ketamine+xylazine and slide preparation with Giemsa stain, experimental animals' chromosomal abnormalities, mitotic index, and blast index were examined. In order to ascertain the protective activity of the plant extract, the mitotic index and blast index are also assessed. The results showed that the exposed group (T1) had a significantly lower mitotic index (the number of cells in mitosis per 1000 bone marrow cells) than the control group (which only drank distilled water). These results showed a significant decrease (p>0.05) of the mitotic index of (the T1) group that received hydroxyurea orally compared with the control group that received distilled water alone. On the other hand; (T2 and T3) groups that received Ginkgo Biloba and omega 3 respectively, revealed a significant increase (P<0.05) in the mitotic index compared with the T1 group that received hydroxyurea alone. The chromosomal aberrations results recorded a significant increase (P<0.05) in a group (T1) that was exposed to HU alone that suggested to be attributed to the genotoxic effect of the drug. On the other hand, a significant decrease (P>0.05) in the mean of chromosomal aberration in both groups (T2 and T3) that pretreated with GB extract and omega3 respectively. HU treatment caused serious CAs formations such as dicentric chromosome, Acentric, deletion, ring, and a high rate of breaks in bone marrow cells. As a results, GbE would be good candidates for administration as a supplement to decrease the Geno-toxicity side effects of a lot of chemotherapeutic agents.

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Section: Groupscontrasting

confidence: 68%

Ginkgo Biloba Extract’s Efficacy to Mitigate the Genotoxicity that Hydroxyurea Induces in Mice

Al-Ameedi¹,

Ayad²,

Mohammed³

et al. 2023

AAVS

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“…This study is part of the results of a complex project devoted to evaluating the ecological situation of populated territories of the Almaty region contaminated by banned organochlorine pesticides over 10 years. The results indicated that organochlorine pesticides and their decay products entered the human food chain and influenced the level of chromosome aberrations and somatic health status [ 15 , 16 , 17 , 18 , 19 , 20 ]. The impact of many factors on health status was estimated in a recently published article [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Case-Control Study of the Association between Single Nucleotide Polymorphisms of Genes Involved in Xenobiotic Detoxification and Antioxidant Protection with the Long-Term Influence of Organochlorine Pesticides on the Population of the Almaty Region

Altynova,

Khamdiyeva,

Garshin

et al. 2023

Toxics

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The association of genetic polymorphisms with the individual sensitivity of humans to the action of pesticide pollution is being actively studied in the world. The aim of this study was a molecular epidemiological analysis of candidate polymorphisms of genes involved in pesticide metabolism, detoxification, and antioxidant protection. Some of the selected polymorphisms also relate to susceptibility to cancer and cardiovascular, respiratory, and immune system diseases in individuals exposed to pesticides for a long time. For a case-control study of a unique cohort of people exposed to organochlorine pesticides for 10 years or more were chosen, a control cohort was selected that matched with the experimental group by the main population characteristics. PCR-PRLF and genome-wide microarray genotyping (GWAS) methods were used. We identified 17 polymorphisms of xenobiotic detoxification genes and 27 polymorphisms of antioxidant defense genes, which had a significantly high statistical association with the negative impact of chronic pesticide intoxication on human health. We also found 17 polymorphisms of xenobiotic detoxification genes and 12 polymorphisms of antioxidant defense genes that have a protective effect. Data obtained added to the list of potential polymorphisms that define a group at high risk or resistant to the negative effects of pesticides.

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Cytome analysis (micronuclei and nuclear anomalies) in bioindication of environmental pollution in animals with nuclear erythrocytes

Cherednichenko,

Magda,

Nuraliyev

et al. 2024

Heliyon

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Сytogenetical bioindication of pesticidal contamination

Cited by 4 publications

References 56 publications

Ginkgo Biloba Extract’s Efficacy to Mitigate the Genotoxicity that Hydroxyurea Induces in Mice

Ginkgo Biloba Extract’s Efficacy to Mitigate the Genotoxicity that Hydroxyurea Induces in Mice

Case-Control Study of the Association between Single Nucleotide Polymorphisms of Genes Involved in Xenobiotic Detoxification and Antioxidant Protection with the Long-Term Influence of Organochlorine Pesticides on the Population of the Almaty Region

Cytome analysis (micronuclei and nuclear anomalies) in bioindication of environmental pollution in animals with nuclear erythrocytes

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