Сравнительное Исследование Микробиоты Эякулята Методом Количественной ПЦР И Культуральным Методом

Ворошилина, Е. С.; Зорников, Д. Л.; Panacheva, EA

doi:10.24075/vrgmu.2019.009

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…Преимущество данного метода заключается в доступности, низкой стоимости, определении не только вида микроорганизма, но и его чувствительности к антибиотикам. Недостатками метода являются длительность выполнения, выявление лишь незначительной части присутствующих в образце микроорганизмов [28].…”

Section: Other Problems Of Endocrinologyunclassified

Features of the intestinal microbiome in patients with thyroid diseases

2022

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Introduction. Today the search and development of new methods for diagnosing organic or functional pathology of the thyroid gland with an asymptomatic or clinically nonspecific course is an urgent task of endocrinology and therapy. The functioning of the thyroid gland and the state of the human intestinal microbiome are dynamically interrelated. One of the considered promising methods is mass spectrometry of microbial markers of the human intestinal microbiota.The purpose of the study. To study the microbial spectrum and characteristics of the biotic environment of the intestine in patients with thyroid diseases.Materials and methods. 21 patients were examined (8 men and 13 women, median age 40.5 [31.75; 54] years, respectively; 15 with thyroid disease, 6 without thyroid disease). In addition to the standard clinical and laboratory-instrumental examination, patients, colon contents were examined by chromatography-mass spectrometry of microbial materials. The work used descriptive non-parametric statistics with subsequent interpretation. Mean values of indicators and their variance are presented as median, upper and lower quartiles. In relation to representatives of the fecal microbiota, in addition to absolute values, the frequency of occurrence of the microorganism within the reference range, moderate or pronounced deviations was taken into account. The determination of the statistical significance of differences in the relative frequency value was carried out using Fisher’s exact test. The presentation of the results is implemented in the form of range diagrams, compactly depicting a one-dimensional probability distribution, tables. Results. Differences in the composition of the microbiota of the control group and the experimental group for Alcaligenes spp., Staphylococcus spp., Megamonas hypermegale, Peptostreptococcus anaerobius were revealed.Conclusions. The development of thyroid gland pathology is accompanied by significant deviations in the composition of the fecal microbiota, determined by mass spectrometry of microbial markers. In persons with thyroid pathology, in the absence of clinically significant external influences on the intestinal microbiome, a pronounced decrease in the level of Alcaligenes spp., Staphylococcus spp., is detected, a tendency to a decrease in the level of Megamonas hypermegale, Peptostreptococcus anaerobius is determined. Further differentiated study of the composition of the intestinal microbiome in patients with thyroid diseases is required, depending on the nosological affiliation and the nature of the endocrine dysfunction.

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Section: Other Problems Of Endocrinologyunclassified

Features of the intestinal microbiome in patients with thyroid diseases

2022

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“…Однако в клинических рекомендациях Европейской ассоциации урологов (European Association of Urology) 2019 г. указано, что для диагностики простатита рутинное бактериологическое исследование эякулята не реко-мендуется в связи с большим количеством ложноположительных результатов [16]. В последние годы для выявления заболеваний придаточных половых желез широко используется не только бактериологический анализ, но и метод полимеразной цепной реакции (ПЦР) [21][22][23][24]. Благодаря более широкому внедрению метода ПЦР появилась возможность детально изучить микробиоту и эякулята, и секрета ПЖ и сравнить результаты рутинного бактериологического анализа и ПЦР в реальном времени (ПЦР-РВ), которые в большинстве случаев совпадают между собой [23,24].…”

Section: Introductionunclassified

“…В последние годы для выявления заболеваний придаточных половых желез широко используется не только бактериологический анализ, но и метод полимеразной цепной реакции (ПЦР) [21][22][23][24]. Благодаря более широкому внедрению метода ПЦР появилась возможность детально изучить микробиоту и эякулята, и секрета ПЖ и сравнить результаты рутинного бактериологического анализа и ПЦР в реальном времени (ПЦР-РВ), которые в большинстве случаев совпадают между собой [23,24]. В научной литературе нам не встретились работы, посвященные исследованию микробиоты секрета ПЖ и эякулята методом ПЦР у пациентов с простатитом IV категории.…”

Section: Introductionunclassified

Comparative analysis of the rate of microorganism detection in the prostatic fluid and ejaculate using real-time polymerase chain reaction in patients with category IV chronic prostatitis

Почерников

Гетьман

Постовойтенко

et al. 2020

Androl. genit. hir.

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The study objective is to compare the rate of detection of various microorganisms in the prostatic fluid and ejaculate using real-time polymerase chain reaction in patients with category IV chronic prostatitis.Materials and methods. Between December of 2016 and July 2019, a prospective study including 81 patients with category IV chronic prostatitis per the National Institutes of Health Prostatitis Syndrome Classification (1999) was performed. The patients referred to the clinic of the Ivanovo State Medical Academy for preconception preparation, infertility or erectile disfunction. At the examination, all patients lacked symptoms characteristic of category II or III chronic prostatitis. Transrectal ultrasound of the prostate, microscopic examination of the prostatic fluid and (or) ejaculate, quantitative examination of urogenital tract microbiota using real-time polymerase chain reaction were performed.Results. Comparison of microbiota of the prostatic fluid and ejaculate showed significant differences in the total amount of bacterial mass: in the prostatic fluid mean titer was 3.7 ± 1.6, in the ejaculate it was 2.6 ± 1.8 (p <0.001). Prostatic fluid contained significantly more of the following microorganisms: Enterobacteriaceae spp./Enterococcus spp., Staphylococcus spp., Streptococcus spp., Corynebacterium, Eubacterium, Anaerococcus (p <0.05). No significant differences in the amounts of other microorganisms were observed.Conclusion. The study demonstrates significant heterogeneity of qualitative and quantitative microbiota content in the prostatic fluid and ejaculate. Supposedly, it can be explained by anatomical and physiological characteristics of the prostate, seminal vesicles and periurethral glands that secrete fluid for the ejaculate. The ejaculate contains less microorganisms compared to prostatic fluid which should be taken into account in differential diagnosis of infections of the urogenital tract.The authors declare no conflict of interest.All patients gave written informed consent to participate in the study.

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The Benefits From Update Laboratory Preanalytical Stage in PCR on the Way to Identify Occult Urological Infections in Male

Pochernikov,

Postovoitenko,

Davidova

2023

Laboratornaya I Klinicheskaya Meditsina. Farmatsiya

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Aim of the study. Assess the diagnostic capabilities of detecting microorganisms in the wild ejaculate and sediment ejaculate. Materials and methods. 42 male volunteers were examined, aged 28 to 69, who were married and had children. Men applied for a preventive examination and to exclude asymptomatic male accessory gland infections. All patients had no active complaints characteristic of inflammatory diseases of the genitourinary tract. The study of the wild ejaculate and sediment ejaculate was performed by PCR real-time on DT-96 amplifiers (NPO DNA-Technology LLC, Russia) using the Androflor? test. Results. The value of the total bacterial mass in the sediment of the ejaculate is not statistically significantly higher compared to the wild ejaculate (p > 0.05). Candida spp. was statistically significantly more common in semen sediment (p < 0.05). In the sediment of the ejaculate were more often identified Enterobacteriaceae spp. / Enterococcus spp., Haemophilus spp., Staphylococcus spp., Streptococcus spp., Bacteroides spp. / Porphyromonas spp. / Prevotella spp. and Anaerococcus spp. (p > 0.05). In 11.9 % of observations, in the absence of bacteria in the wild ejaculate, microorganisms in low titers were identified in sediment ejaculate. Conclusion. According to the data obtained, the performance of the biological sample of the ejaculate sediment is higher compared to the sample of the wild ejaculate. The proposed modification of the preanalytical laboratory stage of the PCR technique, namely the diagnosis of ejaculate sediment using PCR real-time with the Androflor® test, makes it possible to more accurately determine pathogens, including those persisting in the form of biofilms in male accessory gland, even at low concentrations.

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Сравнительное Исследование Микробиоты Эякулята Методом Количественной ПЦР И Культуральным Методом

Cited by 6 publications

References 8 publications

Features of the intestinal microbiome in patients with thyroid diseases

Features of the intestinal microbiome in patients with thyroid diseases

Comparative analysis of the rate of microorganism detection in the prostatic fluid and ejaculate using real-time polymerase chain reaction in patients with category IV chronic prostatitis

The Benefits From Update Laboratory Preanalytical Stage in PCR on the Way to Identify Occult Urological Infections in Male

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