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The participation of various isoenzymes of α amylase in the formation of falling number values of the commodity grain of wheat grown in the Republic of Kazakhstan was investigated. It was found that active isoenzymes α AMY1 and α AMY2 of the embryonic shield were present in the grain with an index over 200. A significant decrease in the falling number depended mainly on the synthesis of α AMY1 and α AMY2 isoenzymes in the aleurone layer. In the grain, isoenzymes with high isoelectric points (p1 ≥ 7.3) were found these isoenzymes belong to α amylase or late maturing or α amylase of practically mature grains. It was dis covered that the exogenous hormone (gibberellic acid) induced synthesis of α amylase isoenzymes of scutel lum, whole caryopses, and aleurone. It was shown that the impact of exogenous gibberellic acid on the activity and structure of α amylase is reduced in grain with a low falling number.

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