We show characteristic morphological changes corresponding to epithelial-mesenchymal transition (EMT) program fulfillment in PANC1 cell line stimulated with TGFβ1. Our results support downregulation of E-cadherin protein. We show 5- and 28-fold increase in SNAI1 and SNAI2 expression levels and 25- and 15-fold decrease in CDH1 and KRT8 expression levels, respectively, which confirms the EMT-program fulfillment. We demonstrate downregulation of expression of pancreatic master genes SOX9, FOXA2, and GATA4 (2-, 5-, and 4-fold, respectively) and absence of significant changes in HES1, NR5A2, and GATA6 expression levels in the cells stimulated with TGFβ1. Our results indicate the absence of induction of expression of PTF1A, PDX1, HNF1b, NEUROG3, RPBJL, NKX6.1, and ONECUT1 genes, which are inactive in PANC1 cell line after the EMT stimulated by TGFβ1.

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In cathodo um nescence spectra of high-quaky GaAs/AI,Ga,.,As m-lti qLantum wells (well thicknesses and barrier compos:tion in the range 2.7 nm < L 6 8.8 nm ( x = 0.32) and 0.12 < x < 1 (L 2 11 nm) respectively) indirect zero-phonon transit ons associated with the bc electron and Ts, valence band s-blevels in tne we Is were observed. Tne experimental findings strongly support a zero-pnonon transition. Us:ng the parameters for the low-temperature excitonic gap E;[L,m ) = 1.81 e V and the L-band etlectve mass in the growth direction m-(GaAs) = 0.105mo the theoretical predictions agree w:th the experimental finaings if the light-hole sublevels are includea 'n the calcLlations (Ls-r;, trans.tions). The interpretation of the observed bands as Lsc-T:: trans.lions yields a sligntly higher excitonic gap 01 E;[L, -CO) = 1.825 eV and an effective mass which decreases with increasing energy.