The aim of the research was to create an effective method for producing transgenic wheat plants suitable for a wide range of promising varieties, both spring and winter crops. The plant material was cultivated at temperatures ranging from 4 to 25°C, either in the dark or in the light, with a 16-hour photoperiod (16/8 - day / night). Osram L36/77 FLUORA and F36W/33 Cool White lamps were used for lighting. The composition of all nutrient media included macroand micro-salts, vitamins B5, phytohormones and carbohydrates. The pH of the medium was adjusted to 5.8 before autoclaving. The medium was sterilized in an autoclave at pressure of 1.2 atmospheres for 15 minutes. An effective method of regeneration of transgenic wheat plants for ballistic transformation has been developed. Plants obtained by this method are phenotypically normal and fully fertile. The transgenic insertion of the target gene is transmitted to the offspring in accordance with Mendel’s laws. The transformation efficiency was high for all the studied varieties and ranged from 1.4 to 7.8%.

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The aim of this research was to study the morphogenetic ability of Taraxacum kok-saghyz root tissues and to optimize the culture medium for the subsequent genetic transformation of plants. The effects of exogenous hormone exposure on survival, in vitro shoot induction, and root formation were studied by using root tissues. For the cultivation the samples of kok-saghyz, the Murashige-Skuga nutrient medium was used as the basis, supplemented with sucrose 20 g/l, vitamins B5 1 mg/l, and also containing agar 5.5 g/l. The pH value is 5.7. For the plant regeneration induction, growth regulators, auxins and cytokinins were added in culture media. In this study, the roots of D 1 mm and two media variants were used for comparison: variant 1. MS + 6-BAP 1 mg/l; variant 2. MS + 6-BAP 1 mg/l + IAA 0.2 mg/l. An effective protocol for the regeneration of kok-saghyz explants was developed. There was a high percentage of regeneration of 87.6% on the medium containing a combination of cytokinin and auxin, as well as a high percentage of direct shoot formation, which was 65.1%, the degree of rooting was 100%, the resulting cultured plant tissues grew well and had a high survival rate after transplantation.