A silver nanoparticle (AgNP) is likely to provide an attractive object for combining a variety of biochemical properties with great therapeutic potential by using radiation. The present study explores the IC 50 value of chitosan-poly (vinyl alcohol) hydrogel (Cs/PVA) and Ag-doped chitosan-poly (vinyl alcohol) (Cs/PVA/Ag) nanocomposite in view of their anticancer application. The aim was to develop (Cs/PVA) based hydrogel synthesized by gamma radiation which could behave both as a nanoreactor for Ag nanoparticle with promising anticancer applications. The (Cs/PVA/Ag) nanocomposite was confirmed by FTIR (Fourier transform infrared) spectroscopy, XRD (X-ray diffraction) and EDX (energy dispersive X-ray) analysis. The anti-cancer activity of the prepared nanocomposites was demonstrated in human liver cancer cell line (HEPG2) and breast cancer cell lines (MCF7). It has significant effects against human liver cancer cell line HEPG2 compared to breast cancer cell line MCF7. Further quantitative analysis on the molecular and protein levels is still required to confirm the impact of chitosan on genotoxic effect before reaching a final conclusion and starting its biomedical application.

Gold nanoparticles (AuNPs) have a biological property that have attracted significant attention in this decade, especially in the field of biomedical application with great therapeutic potential. Additionally, the chitosan molecule appears to be a suitable polymeric complex used in this field. The aim of the current study is to explores the cytotoxicity and the IC 50 value of Au-doped chitosan-poly (vinyl alcohol) (Cs/PVA/Au) nanocomposite that was developed by gamma irradiation with promising anticancer activity. The anti-cancer activity of the prepared nanocomposites was demonstrated in human liver cancer cell line HEPG2 and MCF7 breast cancer cell lines. It has significant effects against both cancer cell line. Concluding that, the future nanomedicine will be impacted greatly by the collaboration of biomedical research and developing nanoparticle therapy in the right directions, which will improve the outcome of cancer patients. It is expected that the next decade will reveal real potential for metal nanoparticles to cross the regulatory barrier into clinical use as effective therapeutics.