The main objective of this study was to produce meat-like process flavouring by using a model mixture based on acid hydrolyzed soybean protein (a-HVP) in addition to xylose, thiamine, cysteine and taurine. Glutamic was the major amino acid in a-HVP followed by aspartic, glycine and alanine. The meat-like process flavour was encapsulated in gum Arabic and stored at room temperature for 6 months. The changes in quality and flavour stability were followed during storage. The results revealed that the roasty and savoury notes were increased whereas chickeny, beefy and grilled meat notes showed significant (P< 0.05) decrease during storage. The gas chromatography-mass spectrometry (GC-MS) analysis showed that 2-methyl-3-furanthiol, the main contributor to beefy note, was the major identified compound in the fresh sample. It showed remarkable decrease after storage for 6 months. The pyrazines and thiazoles showed an opposite trend. A quite agreement was found between the results of the odour profile analysis and those of GC-MS analysis. The results of the present study give important information to the flavourists who are working in field of process flavours, especially meat-like process flavour.
This study aims to evaluate the successive extraction of the active ingredients and their antioxidant activity, anti-arthritic as well as anticancer activity of aerial parts (stem, leaves, and flowers) of Chenopodium murale L. Therefore, C. murale plants were extracted using four solvents with a wide range of polarities: n-hexane, ethyl acetate, methanolic and aqueous extracts. Chemical analysis proved it to be a potential source of protein, fat, carbohydrates, the results showed the percentages were: moisture content (92.45%), ash content (18.19%), crude protein (30.42%), crude lipid (2.86%), and carbohydrate (48.3%) respectively of the C.murale. Methanol extracts showed the highest content of total phenolic (TP), total flavonoid (TF), and total tannin (TT). The active ingredients were assessed as well employing gas chromatography coupled to mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). The HPLC analysis of phenolic compounds confirmed that the methanol extract of C.murale detected high amounts of coumarin, 3, 4, 5 methoxy-cinnamic, and pyrogallol respectively. The ethyl acetate extract of C.murale herb displayed a rise cytotoxic effects on MCF7 (89.30 %), aqueous HCT116 (81%), methanol (60.70%) as well as n-hexane (39.80) respectively at 37°C for 48 h of exposure and concentration 100 µg/ml. In anti-arthritic activity at a dose-dependent, the Ethyl acetate successive fraction proved to be the most significant where it produced a percentage of inhibition ranging from 51.73 to 95.77 % followed by methanol fraction 47.70 to 90.02 % at (P ≤ 0.05), compared to Diclofenac as standard 91.22 to 96.44 %. Thus, our findings highlight the potential of this plant for its possible clinical use to oppose malignancy development against especially breast and colon cell lines with anti-arthritic effects as a bioagent in pharmaceutical industries.
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