This paper describes a method of utilizing UV lasers, given fluorophore (sample). Typically fluorescence lifetimes detectors, and a high-speed digital signal processor (DSP)-based are generally on the order of 1-10 nsec [1]. digitizer to measure the fluorescence lifetime of fluorophores.Fluorescence lifetime measurements require a pulsedThe aim of the design is to create a low cost compact device which guarantees ease of use while not compromising performance. An exitation soucSic t crer izatin turs of ultrafast photomultiplier tube coupled with an excitation light lasers vary, a specific laser is needed which 1nth1s case a filter is used to characterize accurately the fluorescence emission pulsed blue violet laser diode was used ). from the sample. The results obtained from several material These lasers operate at a short wavelength of about 405 nm; specimens are presented. their typical threshold current for a continuous wave operation is about 35 mA, with 55 mA being the maximum. The typical Index Terms-Fluorescence, lifetime spectroscopy, laser continuous wave emission power on this laser iS about 7 mW. applications, high-speed undersampling, swept delay, otnoswv msinpwro hslsri bu W piocations This laser is used to excite the sample and the light from the biodetection. sample is collected using a detector. The most common detectors for low level detection of light are the I. INTRODUCTION photomultiplier tubes. Photomultiplier tubes (PMTs) are extremely sensitive detectors of light in the ultraviolet, visible luorescence spectroscopy has been experiencing and near-infrared. The operating voltage is 15 V along with an remarkable growth over the last two decades where it has adjustable input control voltage. The Hamamatsu H5783 PMT found extensive use in biology and biochemistry [1]. was preferred since it does not have an amplifier allowing it to Applications include identification of tumor-associated detect fast rise and fall decay times. Optical filters were usedproteins, molecular classification for disease diagnosis, to control the spectra of the excitation light and fluorescence identification of therapeutic markers and targets, and basic emission light. These filters help the detector to detect only biological research [2]. Fluorescence measurements have also fluorescence emission light without interference from the helped to identify protein crystals [3]. Fluorescence is widely excitation light. Here in this experiment the photons detected used in biotechnology and analytical applications due to its by the PMT create a voltage signal that can be sampled by a extraordinary sensitivity, high specificity, simplicity, and low high-speed undersampling digitization process. cost compared to other analytical techniques [4]. The paper is organized into five sections. Section II The main focus of the work described here is the explains the design of the fluorescence and electronics development of new adaptive algorithms for fluorescence components. Section III details the method of measuring the lifetime measure...

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