Smallholder farmers in southern African countries rely primarily on cultural control and hoe weeding to combat weeds, but often times, they are unable to keep up with the weeding requirements of the crop because of its laboriousness, causing them to incur major yield losses. Optimisation of crop planting pattern could help to increase yield and suppress weeds and to reduce the critical period of weed control and the weeding requirements to attain maximum yield. Experiments were carried out in Zimbabwe during two growing seasons to assess the effect of maize density and spatial arrangement on crop yield, growth and seed production of weeds and to determine the critical period for weeding. Planting maize at 60 cm row distance achieved higher yields and better weed suppression than planting at 75 or 90 cm row distance. Increasing crop densities beyond the customary three to four plants m−2 gave modest reductions in weed biomass but also diminished crop yields, probably because of increased competition for water and nutrient resources. Maize planted in narrow rows (60 cm) intercepted more radiation and suffered less yield reduction from delaying hoe weeding than those planted in wider rows (75 or 90 cm), and the duration of the weed‐free period required to attain maximum grain yield was 3 weeks shorter in the narrow spacing than that in the 75‐ and 90‐cm row spacings. Weeding was more effective in curtailing weed seed production in the narrow row spatial arrangements than in the wide row planting. The results of these studies show that narrow row spacings may reduce weeding requirements and increase yields.
Sweetpotato (Ipomoea batatas) is an important root crop in Uganda, where yield potential and quality attributes have not been fully exploited due to limited breeding efforts and poor knowledge on the inheritance of some of its agronomic traits. A study was carried out at Makerere University to phenotypically characterise selected sweetpotato cultivars with special reference to sweet potato virus disease (SPVD) resistance, growth habit, flowering and seed set ability, tuber yield and shape, tuber skin and flesh colour, dry matter, starch, sugar and β-carotene content. Twenty cultivars were selected for use in the assessment of their breeding potential and for improvement of yield and quality attributes. Cultivar Munyeera displayed the highest level of SPVD resistance followed by New Kawogo and Polyster as exhibited by relative area under disease progress curves following natural field infection and graft inoculation with SPVD causing viruses, Sweet potato chlorotic stunt virus and Sweet potato feathery mottle virus. Flowering ability was low in some cultivars and a few did not flower at all. Some cultivars e.g. Munyeera, New Kawogo, Silk and Sowola which showed high flowering ability failed to fertilise and set seed when crossed to specific cultivars. Preliminary genetic analysis for yield and quality following crossing elite 7 female and 6 male cultivars in a North Carolina 2 mating design showed wide genetic variability in the F 1 s for the important traits, and heterosis was observed for some traits such as tuber size and number of tubers per plant. Up to five genes may be involved in β-carotene synthesis and probably in combination with other genes in different genetic backgrounds that can modify flesh colour from white to purple. The results demonstrate the possibility to improve sweetpotato for yield and quality using the available germplasm.
In sub-Saharan Africa, sweetpotato (Impomoea batatas L.) production is greatly constrained by sweetpotato virus disease (SPVD) complex. This study was conducted to assess the incidence of viruses in healthy-looking sweetpotato in Uganda and to optimise modern technologies for virus diagnosis. A collection of healthy-looking sweetpotato vines from central Uganda were serologically assayed for sweetpotato viruses and the positive samples were confirmed by RT-PCR. A multiplex RT-PCR assay was optimised for simultaneous detection of Sweet potato chlorotic stunt virus (SPCSV), Sweet potato feathery mottle virus (SPFMV) and Sweet potato mild mottle virus (SPMMV). The use of in vitro thermotherapy was also investigated as a means of eliminating sweetpotato viruses. Four viruses namely SPCSV, SPFMV, SPMMV and SPCFV were detected mostly as single infections in the healthy looking plants. SPCSV (70. 6%) recorded highest incidence followed by co-infection of SPFMV and SPCSV (8.3%). Based on shoot survival and effectiveness of virus elimination, the best results were obtained by exposing plantlets to daily temperature regime of 32 RÉSUMÉEn Afrique sub saharienne, la production de la patate douce (Impomoea batatas L.) est grandement affectée par un complexe de maladies de virus (SPVD). Cette étude était conduite pour évaluer l'incidence maladie des virus sur des boutures apparemment saines de la patate douce en Ouganda et optimiser les technologies pour diagnostic de virus. Des boutures apparemment saines de patate douce collectées au centre de l'Ouganda étaient sérologiquement testées et les échantillons infectés étaient confirmés par RT-PCR. Un essai multiplexe RT-PCR était optimisé pour la detection simultanée du virus du rabougrissement chlorotique de la patate douce (SPCSV), le virus de la marbrure duveteuse de la patate (SPSMV) et le virus de marbrure modérée de la patate douce (SPMMV). L'usage de la thermothérapie in vitro était aussi testé comme moyen d'élimination des virus de la patate. Quatre virus dont SPCSV, SPFMV, SPMMV et SPCFV étaient detectés surtout comme seules infections des plantes apparemment saines. Le SPCSV (70. 6%) avait présenté une incidence élevée, suivi de SPFMV et SPCSV dont le niveau d'infection était le même (8.3%). Basé sur la survie des pousses et l'efficacité de l'élimination de virus, les meilleurs résultats étaient obtenus en exposant les plantules à un regime de température de 32 o C pendant 8 heures sous obscurité et 36 o C pendant 16 heures sous lumière durant quatre semaines. La culture du bout du méristème combinée à la thermothérapie a perimis l'élimination de SPFMV et SPMMV dans 77 % des plants qui étaient au départ infectés avec des virus respectifs. Par ailleurs, l'élimination de SPCSV avait échoué.
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