Crayfish accumulate trace metals, which is why they often serve as environmental indicators. Among heavy metals, Hg, Cd and Pb are considered toxic substances, while Fe, Zn, Cu, Cr, Ni, Mn, V, Al and Li are involved in vital functions, which makes them essential elements in animal organisms. The study objective was to determine concentrations of the above metals in the abdominal muscles, hepatopancreas and exoskeleton of spinycheek crayfish (Orconectes limosus Raff.) and signal crayfish (Pacifastacus leniusculus Dana) from Dga³ Wielki Lake and Pob³êdzie Lake in the Mazurian Lake District, Poland. The analyses were carried out with the use of the following methods: CV AAS (Hg), GF AAS (Cd, Pb) and ICP-AES (Al, Cr, Cu, Fe, Li, Mn, Ni, V, Zn) after wet digestion in HNO 3 and HClO 4 concentrated acid mixture. Regarding mean concentrations (mg kg-1 wet weight) in crayfish, metals formed the following order: