Transporting birds for two to four hours over distances of up to 224 km (140 miles) induced hyperlipacidaemia and hypoglycaemia while the concentration of plasma cholesterol decreased and then increased significantly. These responses were the same in summer and winter. Body temperature was not affected by the experimental conditions. There was a consistent increase in plasma corticosterone which was greater in winter than in summer.

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1. Treating chicks, from 1 d or 5 weeks of age with ACTH three times weekly for 3 weeks depresses growth and causes adrenal hypertrophy at dose rates of 30 IU/kg or more, and depletion of adrenal cholesterol (greater than 10 IU/kg). 2. Treating chicks five times weekly at a dose rate of 30 IU/kg was as effective as a dose of 120 IU/kg three times weekly. 3. Plasma glucose and FFA concentrations of chicks treated thrice weekly with 120 IU ACTH/kg for 3 weeks were within the normal range. 4. Rhode Island Reds were more sensitive to ACTH than Light Sussex. 5. Adrenal cholesterol stores in normal chicks show significant variations with season.

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