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RNA (rRNA) gene. The various multivariate analyses were performed to characterize microbial composition and population. The microbial diversity of samples was analyzed by using phylogenetic tree method. The relationship of an altered endometrial microbiota and implantation success was assessed by ongoing pregnancy. The medium in embryo culture was used as control. RESULTS: The clinical pregnancy analysis showed that 47 IVF patients were pregnant out of 100 patients. Seven among 47 clinical pregnacies failed to reach to the ongoing pregnancy. The microbiome analysis revealed that there was difference in population and diversity of endometrial microbiota between successful ongoing pregnancy and failure patients. Microbes such as Lactoacillus acidophilus, Lactoacillus gasseri, and Brevundimonas diminuta were significantly higher in ongoing pregnant women than those in pregnancy failure. In addition, we found that the endometrium of failure patients possessed much higher population of a microbe belonging to the genera Lactoacillus reuteri. CONCLUSIONS: Our results showed that the microbiome using the tip of embryo transfer catheter in IVF clinics was successfully able to be analyzed and that there was difference of endometrial microbiota between pregnant success and failure. Further studies are needed to examine pathological relationship between pregnant failure and negative dominant microbial population during implantation in the uterus. However, this approach can give us better understanding and insights to increase the pregnancy success rate which is currently at the standstill. References: 1. Moreno I, Codoñer FM, Vilella F, et al. Evidence that the endometrial microbiota has an effect on implantation success or failure.

and elected to undergo single thawed mosaic embryo transfer (STMET) after genetic counseling. When more than one mosaic embryo was available, embryo selection occurred following discussion between the patient, physician, and genetic counselor.RESULTS: A total of 587 frozen single embryo transfer cycles after IVF with NGS occurred during the selection period. STEET occurred in 569 cycles (mean age 35.8). Fifteen patients without euploid embryos elected to pursue STMET following genetic counseling (mean age 37.7). Nineteen mosaic embryos were thawed and transferred in 18 STMET cycles; one embryo did not survive thawing. Nine of 18 mosaic embryos (50.0%) implanted, as evidenced by the presence of a gestational sac, compared to 408/569 euploid embryos (71.7%; p¼0.06). The ongoing pregnancy/live birth rate for mosaic embryos was 4/18 (22.2%), which was significantly less than that for euploid embryos (358/569 or 62.9%; p¼0.001). The spontaneous abortion rate for mosaic embryos was 5/9 (55.6%), significantly higher than for euploid embryos (50/408 or 12.3%; p¼0.006). Of interest, all four embryos that resulted in ongoing pregnancies were identified as mosaic for segmental aneuploidies, while the remaining 12 embryos showed mosaicism for monosomies, trisomies, or segmental aneuploidies.