The ecological situation and modern agro-landscape improvement, soil fertility maintenance and uplifting, formation of ecologically safe agro-ecosystems with germplasm diversity in crop rotation, and cultures-soil conditioners are the focus of the study. Nitrogen is a vital element required in larger quantities by plants; however, lack of it has become a problem in organic farming. The presented study suggests ways organic farming can boost up nitrogen in return to address nitrogen deficiency. The research shows data on the harvest of three-year standing alfalfa and indicators of the efficiency of annual crops’ use in the crop rotation of organic farming. The study findings exhibited that alfalfa accumulates residues of up to 13–17 t ha-1 in three years of standing, containing more than 280–300, 63–80, and 66–120 kg NPK ha-1, respectively. In the efficiency of different crop rotations, the alfalfa accumulated 13 and 20 t ha-1 organic residues in the two- and three-year periods, respectively. More than 11 t ha-1 of green mass containing 65 to 70 kg of nitrogen, 20 to 25 kg of phosphorous, and 55 to 60 kg of potassium accumulates in the soil in welded form. By sowing in good time, mixed with oats, leave the soil with up to 12–15 t of organic matter, containing 190–200, 35–40, and 230–250 kg NPK ha-1, respectively. A pea-oat mixture of about 12.0 t ha-1 of green mass resulted in 132, 46, and 102 kg NPK ha-1, respectively. The latest findings can serve as a basis for the development of new approaches related to the organic farming system in Southeast Kazakhstan.

South and Southeast Kazakhstan underwent soil nitrogen forecasting and diagnosing for nutrition to establish the requirement of nitrogen fertilizers for field crops. The study established the possible use of alkaline hydrolyzable nitrogen as an indicator to provide field crops with available soil nitrogen. Managing the soil organic matter more accurately as the main source of nitrogen helps determine the spatial and temporal variability of its content in each specific field and to use a narrower (oriented to the prevailing soil types) scale of humus availability. The recent studies began in several stages, during 2014–2016, 2015–2017, and 2020–2021, on the irrigated light chestnut calcareous medium loamy non-saline and non-alkaline soil formed at the foothill loess plain of the Zailiyskiy Alatau, Kazakhstan. The nitrogen content of nitrates varied significantly over the years of research. In the control variants (without fertilizers), the recording of the minimum content of N-NO3 took place in 2016 compared with 2014 and 2015. In two consecutive years (2020–2021), 2,816 ha of arable land underwent testing in Almaty, Zhambyl, and Zhetysu regions, Kazakhstan. A total collection of 1,015 soil samples went through analysis, revealing a close correlation between the content of humus and alkaline hydrolyzable nitrogen in the upper soil layer of 0-30 cm. The hydrothermal conditions from a long multi-factorial field experiment data largely affected the intensity of mineralization and immobilization processes of the available nitrogen compounds. The rational use of nitrogen fertilizers makes it possible to increase the field crop yield with good quality and enhance the payback of applied fertilizers, maintaining and improving soil fertility.