A novel immunoliposome delivery system was developed for directed transport into cultured olfactory epithelium cells. Monoclonal antibodies against glial fibrillary acidic protein (GFAP) served as a vector. Fluorescence microscopy showed that the target cells are specifically stained with Dil dye incorporated into liposomal membranes. This transport system holds promise for the delivery of bioactive substances to olfactory epithelial cells and modulation of their capacity to stimulate axonal regeneration.

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Immunoliposomal container system for targeted delivery of minor interfering RNA into Schwann cells was developed. Monoclonal antibodies to myelin basic protein served as the vector. Analysis by sandwich ELISA of myelin basic protein showed specific suppression of the target protein gene expression in Schwann cells after incubation with PEG-conjugated immunoliposomes loaded with minor interfering RNA blocking the synthesis of myelin basic protein. Prospects of effective use of this system for targeting of minor interfering RNA for the treatment of diseases of genetic etiology are discussed.