The
use of microalgae to produce biodiesel is a beneficial and
green substitute for conventional fossil fuels (a major source of
greenhouse gas emissions). Microalgae have several merits, including
their nontoxic nature, fast growth rate, growth not being dependent
on the supply of fresh water, potential for biodegradability, and
not requiring arable land for their growth. The exploration of novel
frontiers to establish large-scale operations is vital for economically
feasible biodiesel production, taking advantage of these favorable
attributes. This review is focused on the recent development of the
enzymatic transformation of microalgal oils into biodiesel. The areas
addressed in this work include (i) microalgae and their lipid content,
(ii) maximization of lipid productivity and lipid extraction, (iii)
biodiesel production by enzymatic transesterification, (iv) enzyme
kinetics, (v) factors affecting enzymatic transesterification, and
(vi) immobilized enzymes and their limitations. In addition, various
aspects of free enzyme and its potential future applications are discussed.
This review also highlights a number of strategies using ionic liquids
(ILs) for the successful production of microalgal biodiesel. The present
study suggests that free enzyme has a greater potential than immobilized
enzyme for the transesterification of bio-oils containing phospholipids
into biodiesel.
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