Detergents and disinfectants for sanitation of milking equipment and dairy equipment must have a wide range of antimicrobial activity and provide a proper cleaning effect. At the same time, they must meet high standards of environmental safety and non-toxicity. The article presents data on the parameters of acute toxicity of the new detergent-disinfectant “Argomol”, based on lactic acid, “Katamine AB”, polyhexamethylene guanidine hydrochloride and colloidal silver solution, as well as the toxicity of the tool, which was determined using the express method of Tetrachymena pyriformis. The acute toxicity of the means and its working 0.5% solution in tentative and detailed experiments on white mice was determined and the median lethal dose (DL50) was determined by the method of G. Kerber. It was found that the DL50 of the means in white mice for intragastric administration when calculated by the method of G. Kerber is 4250 mg/kg body weight, and 0.5% of its solution — more than 11000 mg/kg body weight, i. e. according to SOU 85.2-37-736:2011 “Veterinary drugs. Determination of acute toxicity”, this indicates that the means belongs to the IV class of toxicity, which combines low-toxic substances. The results of research on the effect of the new detergent-disinfectant “Argomol” on the culture of ciliates Tetrahymena pyriformis are presented. It was found that the detergent-disinfectant “Argomol” in concentrations of 0.02–0.5% at exposures of 1–10 min did not show toxic effects on the ciliate Tetrahymena pyriformis. Toxicological studies on ciliates suggest that the detergent-disinfectant “Argomol”, when used in the recommended doses and exposures, can be used for sanitation of milking equipment and dairy equipment, as it is environmentally friendly and low-toxic.