We describe a simple technique for simultaneously imaging multiple layers within an object field onto a single camera. The approach uses a binary diffraction grating in which the lines are distorted such that a different level of defocus is associated with each diffraction order. The design of the gratings is discussed, and their ability to image multiple object planes is validated experimentally. Extension of the technique for spherical-aberration correction is described, and it is shown how the gratings can be used as part of a wave-front-sensing system.
A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving approximately 10 nm z position resolution by parameterising the images with an image sharpness metric.
Measurement of two-dimensional bending in a structural element using intrinsic optical fibre strain gauges would normally require three sensors to be attached to, or embedded within, the structure. The same measurement can now be made using a single multi-core optical fibre, reducing deployment cost and increasing practicality.Fabrication of a novel three-core photonic crystal fibre is described. The ability of the fibre sensor to measure bend in two dimensions is demonstrated in the laboratory using interferometric interrogation at a single wavelength. Deployment of the sensor to measure the deformation of a bridge undergoing loading trials is described.
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