No abstract

Background. Comparative study of clinical and immunological efficiency of systemic [with desloratadine (Aerius®, Bayer n=28)] and topical [with azelastin (Allergodil®, Meda Pharma) n=28] antihistamine single-agent treatment of persistent allergic rhinitis in primary school children (56 children from 6 to 11 years old) was carried out on the basis of monitoring the dynamics of nasal mucosa inflammatory biomarkers (IgE, sIgA, IL-4, IL-8, IL-13, IFN-y, TNFa). Materials and Methods. Questionnaires, selfmonitoring diaries with daily score-based assessment of the symptoms and the score of used pharmacologic agents, nasal discharge and touch smears of nasal cavity mucosa were used as investigative parameters in the study. The study was performed with the permission of the Biomedical Ethics Committee of the State Budgetary Educational Institution of Higher Professional Education «Pacific State Medical University» of the Ministry ofHealth of the Russian Federation. Results. A monthlong therapy with desloratadine enabled clinical control of the symptoms of mild and moderate persistent allergic rhinitis in primary school children in 92,8% of cases and allowed to stop local allergic inflammation intensity as was proved by increasing symptom-free number of days and the fact that symptomatic treatment was not required. The use of desloratadine in the course of the next three months ensured retention of the achieved control. Side and adverse effects in the given group were not registered.

Introduction. The study of genes that control the activity of cytokines is one of the important issues in revealing the pathogenetic mechanisms of allergic diseases. Aims. Determination of the frequency of occurrence of polymorphic gene markers genotypes and characterization of the interleukins 17A, 17F content in blood serum in children with bronchial asthma and allergic rhinitis. Materials and methods. A comprehensive survey of 110 children with allergic diseases of 311 years old and 60 healthy peers. The material for genetic analysis was DNA with the study of mutation points of IL-17A at position 197 (GA) and IL-17F 7488 (TC). The content of IL-17A, IL-17F interleukins was measured by enzymelinked assay. For the statistical analysis we used the Statistica 10, methods for comparing unrelated groups of genotypes distributions to expected values at HardyWeinberg equilibrium with 2. Results. The frequency of occurrence of genotypes in a group of healthy children was as follows: IL-17A (G197A) heterozygous GA (63,333%), homozygous GG (36,667%); IL-17F (T7488C) TT (36,667%), CT (63,333%), genotypes AA and CC werent determined. In children with allergic diseases, all genotypes were determined: IL-17A (G197A), GG (11,818%), AA (19,091%) and GA (69,091%), IL-17F (T7488C), TT (5,454%), CC (35,455%) and CT (59,091%) with the highest specific gravity of the GG genotype and TT. There were no significant differences in IL-17A, IL-17F levels in the blood serum depending on the genotype. Discussion. There were significant differences in the structure of the polymorphisms of the IL-17A, IL-17F genes, blood levels of IL-17A, IL-17F and the risk of the disease in allergic children and healthy peers. The frequency of allergic diseases in children with genotypes AA and TT is statistically higher, but with genotypes GG, CC is statistically lower than with other genotypes.

Expansion of the range of examined T-helper clones has determined more complex immune mechanisms for the implementation of allergic inflammation. Objective. To characterize the parameters and relationships between the serum cytokine profile and T-lympho-cyte subpopulation in peripheral blood of children with bronchial asthma and allergic rhinitis. Materials and methods. 150 children aged between 3-11 years old with bronchial asthma, and allergic rhinitis and 30 healthy volunteers were examined. Immunological parameters were assessed by flow cytometry, the concentration of serum interleukins and IgE were determined by means of enzymelinked immunosorbent assay. Statistical analysis was performed with «Statistica 10» program with a critical level of significance p