PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis

No abstract

RNA extract isolated from spleens of mice immunized with lipopolysaccharide from E. coli induced an immunological memory in normal mice. Application of small amounts of corresponding antigen provoked a specific secondary immune response in RNA primed mice.