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One of the raw milk quality criteria is the count of somatic cells, produced by the cow’s immune system to fight infectious diseases of the mammary gland. The paper presents the analysis of somatic cell count and total bacteria count of milk from cows, vaccinated against mastitis using Startvac vaccine. Tests were performed as a comparison between a dairy unit and a farm under different management conditions and using different milking techniques. Six months after the start of the vaccine application the somatic cell count at the dairy unit decreased by 60 thousand/ml, at the farm by 182 thousand/ml. The agent profile was represented by the following bacteria: Entеrococcus faecium, Staphylococcus aureus, Streptococcus spp., Pseudomonas aeruginosa. Staphylococcus saprophyticus, Staphylococcus epidermidis, Entеrococcus faecalis, Escherichia coli, Bacillus, Lactobacillus were also isolated from udder secretion. After a year of immunization somatic cell count both at the unit and on the farm decreased by 245 and 216 thousand/ml respectively; it is noteworthy that 43.75% of microflora isolated from mammary gland secretion was represented by Streptococcus spp. After two years of the vaccine use the somatic cell count was equal to 371 and 725 thousand/ml at the unit and on the farm respectively. Tests of mammary gland secretions revealed Streptococcus spp. in 27.27% of cases, Staphylococcus aureus and Entеrococcus faecium were isolated in 18.18% of tested samples. It was established that after three years of the vaccine use the major cause of mastitis in cows was Streptococcus spp. (55.00%). During four years of tests, a downward trend in somatic cell count of bulk milk from high producing dairy cows as well as in the number of agents responsible for inflammation in a mammary gland was detected. Somatic cell count of milk from vaccinated animals decreased by 286 and 432 thousand/ml at the unit and on the farm respectively. During the test period Staphylococcus aureus isolation rate declined by 19.41%.

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Relevance. The processes of osseointegration in the area of dental implant placement are accompanied by the development of reactions associ-ated with inflammation and activation of local immune processes in peri-implant tissues. Vitamin D preparations can have a significant local effect on the cells of the innate immune defense of the periodontal tissues.The aim of this work is to study the effectiveness of local application of vitamin D on the immune status of the peri-implant sulcus in rehabilitation of patients with partial secondary edentulous and chronic periodontitis by the method of dental implantation.Materials and methods. Determination of the content of biomarkers (IL-1RA, MCP-1, VEGF, TGF-β1, sTNFR) in the crevicular fluid before the installation of implants and the peri-implantation furrow fluid was carried out by the method of enzyme-linked immunosorbent assay using commercial kits «Vector Best» reagents after 3 and 6 months after surgery. The assessment of the state of the bone tissue was carried out using the Orthopantomograph tm OP300 apparatus (KAVO Dental, Germany). Vitamin D has been used topically at a therapeutic dosage.Results. Vitamin D, by reducing Th-1 and Th-17 cell proliferation and differentiation, blocks the activity of the immune-inflammatory process that occurs during implant placement.Conclusion. Application of colecalciferol in patients with moderate periodontitis leads to the activation of the production of immunoregulatory cyto-kines by the cellular structures of the peri-implantation furrow and can be used in combination with the traditional protocol in this category of patients.

Abstract. Goal: on the basis of the veterinary and laboratory diagnostics department with a testing laboratory, surveillance studies of fodder of vegetable origin from different manufacturers were carried out for the following indicators: urease activity, mass content of soluble protein. An experiment on the activity of urease in full-fat soybean feed was also performed. To assess the quality of heat treatment of soybeans and products of its processing, to analyze the stability of the parameter of urease in fodder soybeans. Methods. These studies were performed in accordance with GOST 13979.9-69; GOST 13979.3-68. Results. As a result of statistical processing, it was revealed that over the past five years, urease activity was above the maximum permissible value in 33 % of feed samples. In some samples of soybeans, this indicator was exceeded by 2.6 times, in the experimental sample of soybeans – by 5.8 times. It was also experimentally confirmed that this indicator is a stable parameter. Additionally, studies of the cumulative mass content of soluble proteins were conducted in these samples. It was found that in 13 % of soybean samples and its processed products, the result of soluble protein was in the range of 68.20–74.50 %, which indicates excessive heat treatment of this raw material. Based on the results of the research conducted, it was found that this feedstock did not always meet the stated requirements. In this regard, the conduct of these studies is necessary for further control of the technological process. Scientific novelty. Currently, the issue of the quality of fodder of vegetable origin and soybean-processed products remains relevant. Full-fat soybean, soybean extracted meal, soybean expeller meal are increasingly used for the preparation of animal feed for farm animals and poultry. In this regard, it became necessary to control the quality and safety of this feedstock.