The investigation was done using bio-formulations containing beneficial microorganisms to assess the impact of beneficial microorganisms on the performance of African marigold cuttings during propagation and their establishment. Significant differences were observed for almost all parameters across different treatments. Observations have shown that the highest survival percentage (78.40%), number of branches per cutting (4.16), number of roots per cutting (34.46), the average length of roots per cutting (6.10 cm.), fresh weight of roots per cutting (0.25 g) and dry weight of root per cutting (0.11g) were recorded with the use of Trichoderma harzianum which gave the best results for all the parameters.

The experiment was conducted in the winter agricultural season 2020 in the greenhouses of the Al-Fares Company in Al-Zubair district, with the aim of investigating one of the important genes responsible for tolerating Armenian cucumber for stress conditions, which is the gene (glutathione S-transferase zeta class-like) in 21 Armenian cucumber cultivars. Isolation of RNA from leaves after 100 days of cultivation at the saline level 5 dSm−1, as well as a control treatment of 1 dSm−1and studying their gene expression using RT-qPCR (Real Time-quantitative Polymerase Chain Reaction) technique. The results showed the excelled of the cultivars under the influence of salt stress, Egypt, Babylon, Mosul (Carmelis), Kirkuk, Diyala and Karbala, as they gave the highest expression of their gene expression (25.12, 21.87, 19.04, 21.87, 19.04 and 20.40), respectively, so they can be considered salt-tolerant cultivars. This is because the expression values for the gene (glutathione S-transferase zeta class-like) were high compared to the rest of the cultivars. As for the cultivars whose sensitivity to salinity was confirmed by the experiment, they are Iranian (Ghani), Italian, Baghdad and Amara. These cultivars gave the lowest gene expression values (2.73, 4.46, 4.14 and 3.49), respectively.

Bacterial blight (BB) incited by Xanthomonas oryzae pv. oryzae (Xoo) is a constant threat to global rice production. Xoo attenuates host defense by directly translocating type III effectors (T3Es) into rice cytosol. The fleeting emergence of new virulent Xoo isolates and subsequent breakdown of resistance in the realm of an erratic climate entails the exploration of befitted resistance to prevent super-races of Xoo from becoming widespread. A comprehensive knowledge of T3Es-mediated transcriptional reprogramming of rice genes in response to Xoo infection remains elusive. Evidently, RNA seq-based transcriptome profiling of rice infected with an Indian virulent Xoo strain Race 4 relative to its T3SS-defective mutant strain (Xoo DhrpX) at early and late hours of bacterial infection yielded an array of differentially expressed genes (DEGs), 49 genes of which were selected for RT-qPCR analysis. The results revealed T3SS-dependent novel defence-related genes belonging to Nucleotide-binding leucine-rich repeat (NLRs), Receptor-like kinases (RLKs), PR proteins, enzymes, cytochrome P450 and secondary metabolites. These genes are direct targets for enhancing BB resistance as well silencing of susceptible targets through gene editing yields durable resistance.