The experiment have been done on the winter farming season 2020 in one of the farms that belongs to Faris company in Basrah governorate, the technique ISSR have been used to study the genetic distance for twenty one isolates of snake melon Cucumis melo var. flexuosus species. The variations between amplified samples have been revealed after running them on a gel of agarose which have been previously stained by ethidium bromide. Five primers which gave varied product on the agarose have been selected. Those five primers produced 713 bands, both primers UBC 813 and UBC 815 showed the higher numbers of bands reached to 177 while the primer UBC862 showed the least numbers of bands (100) and the bands which showed multi-variations showed (46) bands, and the results primers amplification unique bands their number reached to (14) and five of those bands belong to the primer UBC842 while the primer UBC862 produced three bands while the primer UBC807 did not produced any bands. While it has shown 100% polymorphisms with the primers 813, 815, 842 and 862, and the least polymorphism percentage have shown with the primer UBC807 reached 75%. According to the efficiency primers, the highest efficiency percentage shown with the primer UBC813 and 815 reached to 24.82% and the least percentage shown 14.02% by the primer UBC862. Cluster analysis showed the effect on the variance of the studied cultivars.

The experiment was conducted in the winter agricultural season 2020 in the greenhouses of the Al-Fares Company in Al-Zubair district, with the aim of investigating one of the important genes responsible for tolerating Armenian cucumber for stress conditions, which is the gene (glutathione S-transferase zeta class-like) in 21 Armenian cucumber cultivars. Isolation of RNA from leaves after 100 days of cultivation at the saline level 5 dSm−1, as well as a control treatment of 1 dSm−1and studying their gene expression using RT-qPCR (Real Time-quantitative Polymerase Chain Reaction) technique. The results showed the excelled of the cultivars under the influence of salt stress, Egypt, Babylon, Mosul (Carmelis), Kirkuk, Diyala and Karbala, as they gave the highest expression of their gene expression (25.12, 21.87, 19.04, 21.87, 19.04 and 20.40), respectively, so they can be considered salt-tolerant cultivars. This is because the expression values for the gene (glutathione S-transferase zeta class-like) were high compared to the rest of the cultivars. As for the cultivars whose sensitivity to salinity was confirmed by the experiment, they are Iranian (Ghani), Italian, Baghdad and Amara. These cultivars gave the lowest gene expression values (2.73, 4.46, 4.14 and 3.49), respectively.

The experiment was conducted in the winter agricultural season 2020 in the greenhouses of Al-Faris Company in Al-Zubair district The aim of the study is studying the effect of salinity of irrigation water on the mineral leaves content of twenty-one cultivars of Armenian Cucumber(Cucumis melo var. flexuosus), Where the experiment included 42 treatments, which resulted from two main factors, the first one is irrigation water, which are (1and 5 dS.m−1)and the second factor was included 21cultivars, of Armenian cucumber were selected, 16 of which are local and 5 imported. The study showed that the salinity of irrigation water caused a significant increase in the leaves content of potassium and sodium, with an increase of 33.71% and 324.43%, respectively, while irrigation with Naturalwater caused an increase in the percentage of the potassium element to the sodium with an increase reached 330.83%, While the cultivars differed in their content of mineral elements, where the Tikrit cultivar excelled with the highest content of phosphorous and potassium amounted to 0.2299% and 6.820%, respectively. While the Diyala cultivar gave the highest leaves content of sodium 5.548%) and the Karbala cultivar excelled and gave the highest percentage of potassium to sodium in the leaves of 5.681%), while the Italian cultivar recorded the lowest percentage of phosphorus in the leaves 0.0201, the Diwaniyah cultivar gave the lowest percentage of potassium in leaves, reaching 4.240%, and the cultivar Amarah gave the lowest percentage of sodium in the leaves, reaching 2.218%.Where the Mosul cultivar (Abu Akaf) gave the lowest average of potassium to sodium ratio in the leaves when it reached 1.209%).