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Background: Grapevine is an important perennial crop worldwide, consumed as fresh or dried (raisins) fruit. Grapevines are exposed to a variety of abiotic stresses during their growth. Water shortage is one of the primary stressors and severely restricts the development of the grape industry. Arbuscular mycorrhizal fungi (AMF), a kind of beneficial soil microorganism, can create a symbiotic association with plant roots forming arbuscular mycorrhizas (AMs), which play a role in the regulation of plant growth. Methods: This research was accomplished in order to investigate the effect of 4 species mycorrhizal fungi on grapevine ‘Asgari’ cultivar under water stress conditions. We use of 3 irrigation regime that consist: 100% (as control), 70 and 40 % of field capacity. As well as, mycorrhizal treatments include: non-use of mycorrhizal (NM) and use of mycorrhiza (M) (Glomus mosseae, G. intraradices, G. etunicatum and G. verciform). Result: The results indicated that shoot and root dry weight, pigments (total chlorophyll and carotenoids), relative water content (RWC), P, Mg2+ and Ca2+ under water limitation decreased but electrolyte leakage (EL), proline and total soluble sugars (TSS) increased chlorophyll content, RWC and nutrient (P, K+ and Ca2+) in M plants was higher than NM plants. Generally, the use of mycorrhiza fungi in this experiment reduced the harmful effects of water stress. Our results shown that G. verciform and G. etunicatum were better for symbiosis with “Asgari” grapevine under water limitation.