The complexes of iron (II) and nickel (II) with schiff base derived from benzoin and 2-amino benzoic acid have been prepared. Solubility, melting point, decomposition temperature, conductance measurement, infrared (IR) and UV-Visible spectrophotometric studies were used in characterizing the compounds. The melting point of the schiff base determined is 120 o C. The decomposition temperatures of iron (II) and nickel (II) complexes are 152 o C and 155 o C, while the molar conductance values are 11.2 and 10.7 ohm-1 cm 2 mol-1 , respectively. The UV-Visible spectrophotometric analysis revealed 1:1 (metal-ligand) stoichiometry for the two complexes.

A New Schiff base (o-hydroxy benzylidene trimethoprim) derived from trimethoprim (known as a drug) was used as a ligand in this study, has been synthesized by condensation reaction from equal amounts of ortho hydroxybenzaldehyde and trimethoprim. This was then used for the preparation of new complexes by the reaction with Zinc and Manganese salts. The synthesized complexes were in the formula [M(OHTMP)2]X2 in the natural medium and [M(OHTMP)2-H] in basic medium (M=Zn, Mn, OHTMP = o-hydroxy benzylidene trimethoprim X= CH3COO, NO3, Cl2). The prepared complexes were diagnosed and characterized by employing FT-IR, 1H- NMR, CHN element analysis, molar conductivity, Molar refraction and flame atomic absorption techniques. Spectroscopic results proved the chelation behavior of this ligand. It coordinated to the metal ions as a tridentate ligand, via the imine nitrogen, azo methine, and phenolic oxygen atoms. According to the obtained results, octahedral geometry has been suggested for prepared complexes. The biological activities of the prepared ligand and complexes against Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Klebsiella spp.) have being conducted. Both, the ligand and complexes showed anti-bacterial activities, beter than that of the Trimethoprim.