Dental illness is a foremost health problem upsetting childhood, and this action severely causes sickness which is caused by emerging pathogen Lactobacillusacidophilus. So plaque samples were collected from diverse dental clinics around Tirupur Dt. In this study, 20 MDR isolates were isolated and identified by biochemical analysis. Additionally, the decay pathogen Lactobacillus acidophilus was assayed against Levofloxacin, Moxifloxacin, Clindamycin, Vancomycin, Ampicillin, Chloramphenicol, Tetracycline and Novobiocin. Among these strains 0%, 0%, 26%, 34%, 22%, 2%, 16%, 16% and 6% were found to exhibit resistance to four groups of antibiotics. Currently, the extension of resistance to contemporary antimicrobials has demand to search for innovative anti-therapeutic agent. Moreover, several studies mostly deal with marine sponge to explore the therapeutic drug and this potential role in the manufacture of metabolites is becoming a driving area for exploring a rich source of novel compounds that are of prospective interest to mankind for diagnosis of tooth decay. Different concentration of sponge 50μg, 100μg, and 150μg were prepared with DMSO, and well diffusion assay was used. The result indicated that the maximum and minimum inhibition of 22 mm and 16 mm was observed against the isolate LAVG07 and LAVG08. This research proved that the marine sponge extract shows the prominent antimicrobial activity against decay causing organism and it may be broadcast to be a successful approach to come to blow promising multi challenging clinical drugs in the future.

Dental illness is a foremost health problem upsetting childhood and this action, severely causes sickness which is caused by emerging pathogen Lactobacillus acidophilus. So plaque samples were collected from diverse dental clinics around Tirupur Dt. In this study, 20 MDR isolates were isolated and identified by biochemical analysis. Additionally, the decay pathogen Lactobacillus acidophilus was assayed against Levofloxacin, Moxifloxacin, Clindamycin, Vancomycin, Ampicillin, Chloramphenicol, Tetracycline and Novobiocin. Among these strains 0%, 0%, 26%, 34%, 22%, 2%, 16%, 16% and 6% were found to exhibit resistance to four groups of antibiotics. Currently, the extension of resistance to contemporary antimicrobials has demand to search for innovative anti-therapeutic agent. Moreover, several studies mostly deal with marine sponge to explore the therapeutic drug and this potential role in the manufacture of metabolites is becoming a driving area for exploring a rich source of novel compounds that are of prospective interest to mankind for diagnosis of tooth decay. Different concentrations of sponge 50μg, 100μg, and 150μg were prepared with DMSO, and well diffusion assay was used. The result indicated that the maximum and minimum inhibition of 22 mm and 16 mm was observed against the isolate LAVG07 and LAVG08. This research proved that the marine sponge extract shows the prominent antimicrobial activity against decay causing organism and it may be broadcast to be a successful approach to come to blow promising multi challenging clinical drugs in the future.

The scientifi c evaluation, validation and authentication of medicinal plants are highly essential and key steps to achieve effective phytochemicals and drugs for disease prevention. The checklist of medicinal plants especially mangroves along with their scientifi c validation is still biased. The traditional methods are poor, time consuming as well as less scientifi c, so there is a need to used emerging technological knowledge and sophisticated analytical methods to fulfi ll this knowledge gap. Therefore, present study is an attempt to study the phytochemistry and recognize HPTLC pattern of Avicennia marinastem and spectrum analysis of resolved compounds in three different extracts. As a result, presence of different class of phytochemicals were detected. HPTLC determination densitometry analysis of 10 μl sample size under UV-258 nm of stem extract -1 (5 % NH 3 ) showed the presence of 2 components with Rf values ranging from 0.76-0.90; the stem extract -2 (methanol alone) revealed the presence of 4 components with Rf values in the range of 0.03 -0.90; and the stem extract -3 (5 % HCl) revealed presence of 7 components with Rf values in the range of 0.03 -0.90. As result, extract -3 (Slightly acidic) gave maximum number of separated compounds and characteristic fi ngerprinting pattern and may be useful as a biochemical integrity check marker for this plant as well as to assess the diversity and further characterization of phyto-compounds present in Avicennia marina stem.