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Nanostructured copper oxide (CuO) thin films were prepared by spray pyrolysis technique. X-ray diffraction was used to investigate the structural properties. Surface morphology was studied using scanning electron microscopy. Microstructure was studied using a transmission electron microscope, and energy-dispersive X-ray analysis was used to determine the elemental composition of prepared nanostructured CuO thin film. Gas-sensing performance was conducted using static gas-sensing system, at different operating temperatures in the range of 200°C to 400°C for the gas concentration of 100 ppm. The maximum sensitivity (S = 872) to H 2 S was found at the temperature of 250°C. Quick response (2 s) and fast recovery (5 s) are the main features of this film.