In ovo injection of nutrients can modulate the embryo’s physiological responses against aflatoxin B1 (AFB1) embryotoxicity. This hypothesis was tested using in ovo injection of Arctostaphylos uva-ursi (Ar. uu.) methanolic extract. The total polyphenols, total flavonoids, total antioxidant capacity, and GC-MS analysis were all assessed in the Ar. uu. methanolic extract. A total of 180 ten-day-old embryonated eggs were distributed into six groups of 30 replicates each. The first group was used as a control (non-injected), and the second, third, fourth, fifth, and sixth groups were injected with 10 µ double-distilled water (DDW), 500 µL methanol, 0.01 g Ar. uu./500 µL methanol, 50 ng AFB1/10 µL DDW, and 50 ng AFB1 in 10 µ DDW + 0.01 g Ar. uu./500 µL methanol, respectively. The relative embryo weight, residual yolk sac weight, tibia length and weight, and survival were recorded. Total and differential leukocytes, oxidative stress, and humoral immune responses were observed. The residual yolk sac was lower (p < 0.05) in the Ar. uu. group than other groups. The embryonic growth (tibia weight and length) was enhanced in AFB1 + Ar. uu.-injected embryos compared with those injected with AFB1 alone. In conclusion, in ovo injection of Arctostaphylos uva-ursi could modulate AFB1-induced toxicity in chicken embryos.

The objective of the current study was to evaluate the impacts of feed restriction (FR) for 3 hours and/or dietary supplementation with vitamins and minerals on growth, mortality rate, some carcass traits, and serum biochemistry indices of broiler chicks under heat stress in the open housing system. A total of 240 one-week old broilers were weighed and randomly assigned into 4 equal experimental treatments. Treatment 1 (T1) was fed ad libitum on basal diets, T2 birds were fed on basal diets with FR, T3 birds were fed ad libitum on basal diets with vitamin C (200 mg) + vitamin E (200 mg) + Cr (1.5 mg ) + Zn (100 mg) per kg diet, and T4 birds were fed on basal diets with FR and combined supplementation of vitamins and minerals. It was found that growth parameters improved in T2, T3, and T4, while the mortality rate was significantly reduced compared with control. FR and feed additives (T4) revealed marked increases in serum total protein and a decrease in total serum cholesterol. It was concluded that combined effect of FR and feed additives could significantly alleviate heat stress effects for broilers by improving growth performance and decreasing mortality rate.

This experiment was aimed to determine the best strategies for alleviating the detrimental effects of heat stress by feed restriction (FR) in hot times and dietary supplementation with Vit.+Min. (vitamins and minerals) by their impacts on growth parameters, mortality rate, serum biochemistry indices and carcass traits of broiler under summer conditions in the open-sided house. A total of 420 broilers of Ross 308 (unsexed 7-day-old) were equally divided into 7 treatments (T). Throughout the experiment, chicks in T1 (control) fed ad libitum on basal diets, FR was applied in T2, T3 and T4 as followed 2, 3, 4 hours (h) without feeding respectively. Supplemental vitamin C (Vit.C) + vitamin E (Vit.E) + chromium (Cr) + zinc (Zn) as followed 100+100+0.5+50 (T5), 150+150+1.0+75 (T6), 200+200+1.5+100 (T7) mg/kg diet respectively. It was found that FR and feed additives improved body weight, weight gain, and significantly decreased feed conversion, mortality rate, and abdominal fat of stressed broiler. Applying FR (3, 4 h) and supplementation with Vit.+Min. (T6, T7) significantly increased total serum protein and statically decreased total serum cholesterol. Overall results conducted that FR for 3 h (T3) and dietary supplementation (T7) have the most significant results which indicate the vital role of FR and supplementation with Vit.+Min. in stressed broilers.

The inclusion of dry corn barda in diets helps to solve the problem of obtaining inexpensive feed protein for broilers, but the effectiveness of its use in different doses has not been studied good enough. The use of different intermittent feeding programs, as of a intermittent feeding mode can reduce the severity of heat stress in broiler chickens. However, the optimal duration of the break in feeding broiler chickens in the hottest daytime hours is not established in detail. The purpose of the researches was to determine the effect of diets with dry corn barda and intermittent feeding mode with the addition of the complex of trace elements (chromium, zinc) and vitamins (ascorbic acid, antisterility vitamin) on the productive and biological characteristics of broiler chickens at high air temperature in the hot arid climate. The productive and biological trats of broiler chickens have been determined when using 5, 10, 15 % dry corn barda in diets. Data on the productive and biological parameters of broiler chickens of the cross Ross-308 have been obtained when using intermittent feeding mode of different duration (2, 3, 4 hours). New data have been obtained on the productive and biological parameters of broiler chickens cross Ross-308 when using additives in feed of different doses of trace elements (Cr, Zn) and vitamins (C, E). The optimal parameters of the duration of intermittent feeding mode and the dose of trace elements (Cr, Zn) and vitamins (C, E) added to the feed have been revealed. It has been found that the inclusion of dry corn barda in the diet of broiler chickens and the influence of intermittent feeding mode with the addition of the complex of trace elements (Cr, Zn) and vitamins (C, E) in the feed improves the functional state of the body and increases the productivity of broiler chickens at elevated air temperature in the hot arid climate.

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