Kuwaiti desert samples contaminated with crude oil contained Bacillus stearothermophilus strains capable of growth on crude oil as a sole source of carbon and energy, obligately at high temperature. No thermophilic oil utilizers were present in water samples collected from the Arabian Gulf. Most of the desert strains had an optimum temperature of 60°C and grew best on pentadecane (C15), hexadecane (C16) and heptadecane (C17). n-Alkanes with shorter and longer chains, n-alkenes, and aromatic hydrocarbons were less readily utilized.
Candida albicans KTCC 89062 grown on nalkanes showed higher levels of sterol content as compared to glucose-grown cells. Certain sterols, such as lanosterol, were significantly reduced in cells grown on n-alkanes, while others, such as ergosterol, increased in these cells. Sterol fractions declined as the chain length of the n-alkanes increased. Ergosterol supplementation of the chemically defined medium showed an increase in the uptake of dodecane (C12) by cells grown on such medium. Increase in the concentration of ergosterol supplementation resulted in an increase in Clz uptake. The uptake of C12 was not stimulated by ergosterol supplementation in the case of non-viable yeast cells.
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