Crude oil and hydrocarbon-degrading strains of Rhodococcus rhodochrous isolated from soil and marine environments in Kuwait

Sorkhoh, N.A.; Ghannoum, Mahmoud A.; Ibrahim, A. S.; Stretton, R.J.; Radwan, S. S.

doi:10.1016/0269-7491(90)90162-6

Cited by 173 publications

(85 citation statements)

References 11 publications

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“…This may be due to differences in nitrogen uptake mechanisms or to competition from co-occurring species (17,22). Bacteria also vary in the rate and efficiency with which they degrade alkane substrates (33,38,43), so an understanding of which species are most effectively incorporating added nutrients in contaminated Arctic soils will help in determining whether these treatments can be further optimized for more rapid mineralization of hydrocarbons.…”

mentioning

confidence: 99%

Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [ ¹⁵ N]DNA-Based Stable Isotope Probing and Pyrosequencing

Bell

Yergeau

Martineau

et al. 2011

Appl Environ Microbiol

123

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Arctic soils are increasingly susceptible to petroleum hydrocarbon contamination, as exploration and exploitation of the Arctic increase. Bioremediation in these soils is challenging due to logistical constraints and because soil temperatures only rise above 0°C for ϳ2 months each year. Nitrogen is often added to contaminated soil in situ to stimulate the existing microbial community, but little is known about how the added nutrients are used by these microorganisms. Microbes vary widely in their ability to metabolize petroleum hydrocarbons, so the question becomes: which hydrocarbon-degrading microorganisms most effectively use this added nitrogen for growth? Using [15 N]DNA-based stable isotope probing, we determined which taxonomic groups most readily incorporated nitrogen from the monoammonium phosphate added to contaminated and uncontaminated soil in Canadian Forces Station-Alert, Nunavut, Canada. Fractions from each sample were amplified with bacterial 16S rRNA and alkane monooxygenase B (alkB) gene-specific primers and then sequenced using lage-scale parallel-pyrosequencing. Sequence data was combined with 16S rRNA and alkB gene C quantitative PCR data to measure the presence of various phylogenetic groups in fractions at different buoyant densities. Several families of Proteobacteria and Actinobacteria that are directly involved in petroleum degradation incorporated the added nitrogen in contaminated soils, but it was the DNA of Sphingomonadaceae that was most enriched in 15 N. Bacterial growth in uncontaminated soils was not stimulated by nutrient amendment. Our results suggest that nitrogen uptake efficiency differs between bacterial groups in contaminated soils. A better understanding of how groups of hydrocarbon-degraders contribute to the catabolism of petroleum will facilitate the design of more targeted bioremediation treatments.

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mentioning

confidence: 99%

Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [ ¹⁵ N]DNA-Based Stable Isotope Probing and Pyrosequencing

Bell

Yergeau

Martineau

et al. 2011

Appl Environ Microbiol

123

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“…The number of indigenous bacteria in the seawater samples was determined using spread plate method on nutrient agar (Oxoid, United Kingdom) and on solid minimal salt media (MSM) supplemented with 1 % of crude oil. The MSM agar was prepared by adding 1.5 % of agar into MSM media and autoclaved at 121°C for 15 min (Sorkhoh et al 1990). After cooling down to 60°C, 1 mL (v/v) of crude oil was added into the MSM and poured into petri dishes and left to solidify.…”

Section: Sampling and Analyses Of Seawatermentioning

confidence: 99%

Self-immobilised bacterial consortium culture as ready-to-use seed for crude oil bioremediation under various saline conditions and seawater

Kee

Hazaimeh

Mutalib

et al. 2014

Int. J. Environ. Sci. Technol.

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Biodegradation of crude oil hydrocarbon by microorganisms in seawater is generally slow because of the harsh environmental condition due to high salinity. The aim of this study was to compare sawdust (SD) and oil palm empty fruit bunch wastes as suitable carrier material to immobilise hydrocarbon-degrading bacterial consortium culture to accelerate and improve crude oil degradation in seawater. The consortium culture was found able to tolerate salinity up to 3 %, where the degradation of crude oil was not inhibited (p [ 0.05). In artificial seawater, suspension of bacterial consortium culture was able to degrade 83.3 ± 3.00 % of crude oil within 8 weeks, which indicated the possibility of using consortium culture in seawater. When tested in seawater, suspension of consortium culture managed to degrade 47.7 ± 1.53 % of crude oil in 8 weeks. In order to improve the performance of consortium culture, immobilisation of consortium culture onto SD and oil palm empty fruit bunch was successfully undertaken when formation of biofilm layers was observed under scanning electron microscope. Immobilising consortium culture onto oil palm empty fruit bunch and SD was shown to increase crude oil biodegradation to 68.7 ± 4.04 and 62.3 ± 5.51 % in 8 weeks, respectively. This study demonstrated immobilisation of consortium culture onto SD and oil palm empty fruit bunch can be utilised as ready-touse seeds to improve and accelerate crude oil biodegradation in seawater.

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“…Teste realizado em laboratório (plaqueamento em meio agar chromocult ® ), cuja finalidade consistiu na verificação da presença de bactérias do grupo coliformes totais e a bactéria Escherichia coli, mostrou que os três pontos de coleta apresentavam E. coli, sendo que somente no ponto SB1 foram verificados os coliformes totais. A bactéria E. coli em outros estudos (Sorkhoh et al, 1990;Al-Hadhrami et al, 1995) foi considerada como capaz de biodegradar hidrocarbonetos, contudo, a presença dos coliformes é um forte indício da infiltração de esgoto doméstico. Outros parâmetros (Tabelas 1 a 3) também são indicativos quando são comparados os valores obtidos para SB2 relativamente à SB1, que estão situados proximamente, por exemplo, menor concentração de oxigênio dissolvido, e maiores concentrações de sódio, fosfato, sulfato e nitrato.…”

Section: Resultsunclassified

Monitoramento de indicadores geoquímicos e avaliação de biodegradação em área contaminada com óleo diesel

Mariano

Angelis

Bonotto

2007

Eng. Sanit. Ambient.

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A biorremediação natural da água subterrânea contaminada com óleo diesel em um posto de combustíveis foi avaliada mediante o monitoramento de indicadores geoquímicos e ensaios laboratoriais de biodegradação. Durante um período de dois anos foram realizadas quatro coletas de água subterrânea para a avaliação de diversos parâmetros físico-químicos e a concentração de hidrocarbonetos. As análises microbiológicas consistiram na contagem de bactérias heterotróficas totais, na verificação da presença de bactérias hidrocarbonoclásticas e no teste de biodegradabilidade utilizando o indicador redox DCPIP. Foi possível identificar fatores que corroboram o emprego da técnica de biorremediação natural para o tratamento da água subterrânea, contudo, a presença de hidrocarbonetos retidos no solo representa uma fonte contínua de contaminação, por isso se faz necessário o monitoramento a longo prazo.

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Crude oil and hydrocarbon-degrading strains of Rhodococcus rhodochrous isolated from soil and marine environments in Kuwait

Cited by 173 publications

References 11 publications

Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [ ¹⁵ N]DNA-Based Stable Isotope Probing and Pyrosequencing

Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [ ¹⁵ N]DNA-Based Stable Isotope Probing and Pyrosequencing

Self-immobilised bacterial consortium culture as ready-to-use seed for crude oil bioremediation under various saline conditions and seawater

Monitoramento de indicadores geoquímicos e avaliação de biodegradação em área contaminada com óleo diesel

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Crude oil and hydrocarbon-degrading strains of Rhodococcus rhodochrous isolated from soil and marine environments in Kuwait

Cited by 173 publications

References 11 publications

Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [ 15 N]DNA-Based Stable Isotope Probing and Pyrosequencing

Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [ 15 N]DNA-Based Stable Isotope Probing and Pyrosequencing

Self-immobilised bacterial consortium culture as ready-to-use seed for crude oil bioremediation under various saline conditions and seawater

Monitoramento de indicadores geoquímicos e avaliação de biodegradação em área contaminada com óleo diesel

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Product

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Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [ ¹⁵ N]DNA-Based Stable Isotope Probing and Pyrosequencing

Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [ ¹⁵ N]DNA-Based Stable Isotope Probing and Pyrosequencing