It was found that at low temperature (6-8 degrees C) in the absence of nitrogen supply and at the presence of phosphate ions in the medium, Yersinia pseudotuberculosis and Listeria monocytogenes are able to actively synthesize reserve substances as polyphosphates. Most of the bacterial polyphosphates are alkali-soluble, especially at the preliminary stage of cell growth (lag-phase). This is proved by electron microscopic studies of ultrastructure of model microorganisms. During a long starvation period under conditions of carbon and energy source deficit, L. monocytogenes and Y. pseudotuberculosis consume this biopolymer for biosynthetic and bioenergetic processes.
Ultrastructural changes in the population of pathogenic Yersinia pseudotuberculosis inhabiting a model soil ecosystem for a long time were studied. Changes in the bacteria were mainly adaptive until the 8th month of the experiment, their capacity to binary division was preserved. After 9 months cell structure changed: extracellular amorphous matrix appeared, probably due to increased mucus production.
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