Es werden zwei Methoden zur Testosteronbestimmung im Plasma bzw. Urin beschrieben, die auf dem Prinzip der kompetitiven ProteinBindungsmethode beruhen. Bei der Plasma-Bestimmungsmethode wird das proteingebundene Testosteron vom freien Testosteron mittels Sephadex abgetrennt, während bei der Urin-Bestimmungsmethode diese Trennung mittels Florisil erreicht wird. Wir studierten die Plasmabindungskapazität für Testosteron in Abhängigkeit von der Plasma-Verdünnung und den Einfluß der Sephadex-bzw.
Determination of testosterone in plasma and urine by the competitive protein binding methodMethods are described for the determination of testosterone in plasma and urine, based on the principle of the competitive protein binding method. For plasma, the protein bound testosterone is separated from free testosterone with Sephadex, while Florisil is used for this separation with urine. The plasma binding capacity of testosterone was studied in relation to the plasma dilution; the influence of quantity of Sephadex or Florisil on the exchange reaction was studied. The criteria for reliability are reported. Studies on the plasma of 15 women (20-30 years) gave testosterone values of 20-100 ng/100 m/. The urinary excretion values for 20 women (20-30 years) lay between 2.0 and 28 /wg/24 hr.
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