The levels of nitrate in denitrifying cells of Paracoccus denitrificans were determined by centrifugation through silicone oil into phosphoric acid and ion-exchange HPLC analysis of the cell lysates, using [14C]sucrose to correct for the trapped external medium. Introduction of oxygen brought about a significant upward shift in the intracellular nitrate concentration. This result calls into question the current thinking that oxygen blocks nitrate respiration primarily due to the inhibition of nitrate transport into the cell.
A short discussion of the history of the ore mining in St. Joachimsthal is presented, emphasizing its role in the discovery and production of radium, production of uranium dyes and in the development of radiotherapy. The historical procedures used in the production of uranium and radium in St. Joachimsthal are also briefly outlined.
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