The present study aimed to evaluate efficacy of microvesicles (MVs) derived from mesenchymal stem cells on healing of experimentally induced full-thickness skin wound in donkeys.6 adult healthy donkeys were subjected to two full thickness skin wounds on the back region.Hence; two groups were evaluated; wounds on the left side were treated with injection of saline solution 24 hrs post wounding (PW)and repeated at day 7 PW as control group and wounds on the right side treated with MVs solution 24 hrs PW and repeated at day 7 PW. Wounds were clinically for determination of wound surface area and percentage of wound contraction. Histopathological evaluation was performed at days 21 and 42PW. Immunohistochemical evaluation was performed at 21 days PW for detection of the activity against CD31 and alpha smooth muscle actin (α-SMA) markers. The wound surface area progressively decreased in both MVs and control groups and it was significantly lower in MVs group than the control group. The percentage of wound contraction was significantly higher in MVs group than the control group. Histopathological evaluation revealed better findings in MVs group compared to the control group. MVs group showed high immunreactivity against both CD31 and α-SMA antigensand the control group showed no immunreactivity against both antigens.In conclusion, mesenchymal stem cells derived microvesicles could promote neovascularization, re-epithelialization and collagen deposition at wound sites and thus accelerate wound healing in experimentally induced full thickness skin wounds in donkeys when compared to the control group.