The present study was conducted during the period from October 2013 to May 2014 in Wasit Province to detect the T. gondii in women using molecular and serological methods. Five hundred blood samples and 8 placenta specimens were collected from suspected women. The sera samples were separated and examined by ELISA to detect Toxoplasmosis serologically. In addition 89 blood samples and 8 placental tissue samples were subjected to polymerase chain reaction (PCR) technique to detect for molecular identification. The Serological results showed that 17.8% of women were positive for the private screening ELISA detects toxoplasmosis (17 % with chronic infection and 0.8% with acute infection). Moreover, it was noted that the highest rate of infection was in women who ranged in age between 20-29 years, reaching 19 .9%, but with no significant difference (P>0.05) between the ages studied. The study indicated a lack of months effect on the distribution of parasite infection rates where these different months recorded relatively close rate ranged between 14.45%-23.07% with no significant difference (P<0.05). Regarding to polymerase chain reaction test, when a fragment of 399bp was amplified from B1 gene, the result showed that 6.74% of blood samples and 100% of placental tissue samples were positive to this test. In Conclusion the Toxoplasma infection in women was relatively high in Wasit Province.

The aim of present study was to detect the seroprevalence of Ostertagia ostertagi specific antibodies in cattle milk samples in Wasit province, by using the indirect ELISA test for first time in Iraq. For this purpose, an overall 368 dairies cow was submitted for study and the results were revealed that 51 (13.86 %) of tested cows were positive, and the mean optic density ratios (ODRs) of ELISA test values in seropositive cattle were 0.58. Also, this study aimed to investigate an association of seropositive results with some epidemiological risk factors. Hence, the positive results, according to these factors, were as follow: in milk production factor, 6.32 % for ³18 liters/day group, 14.29 % for ³10-18 liters/day group, and 25 % for < 10 liters/day group; in age factor, 13.41% for ³3-6 years group, and 14.75% for >6 years group; in breed factor, 18.27% for local breed group, 12.17% for cross-breed group, and 12% for pure breed group; in farm management factor, 21.35% for bad management group, and 5.68% for good management group; and in herd size factor, 11.59% for <25 (cow/herd) group, and 17.78% for ³ 25 (cow/herd) group. Statistically, the significant differences (P£ 0.05) were observed among related groups of milk production, breed, husbandry management, and herd size factors; while it’s not reported among groups of age factor.

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