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The main goal of all production operators is maximizing production and net profit out in a very safe and environmentally controlled manner. When producing difficult fluids from marginal, mature and brown onshore oil fields, Sucker rod (Beam and Progressive Cavity) Pumping systems are typically the most common artificial lift methods used. The primary challenge is to extend the lifting equipment run life, especially the downhole components. For years, operators have been looking for a reliable and accurate way to automatically control these types of lifting systems to improve their reservoir recovery factor by maximizing production. The best-proven way for operators to make proactive decisions for well / field optimization, is to have a fully automated closed-loop monitoring and control solution for the entire field of artificially lifted wells. This paper will show how the well automation and real time downhole measurements are used in real time to control and optimize the operation parameters and well production to obtain maximum benefits. Some case histories for Beam and Progressive cavity pumping systems from different oil fields will presented.

Concentrating on the interferon alpha 2b gene (INF 2b), a protein having antiviral and anticancer properties, could be beneficial therapeutically. It is advised to conduct an experimental examination of the target protein before having it produced on a big scale. The bacterial host is a good option since it allows us to gain a thorough understanding of the topic. The INF-2b sequence used in this study was obtained from the NCBI gene data library, and following optimization, it was cloned and expressed in pET28a+.Escherichia coli was chosen as the prokaryotic expression system for the target protein's initial analysis. Bacterial cells expressing the pET:IFN construct were IPTG-induced to produce the protein, which was then resolved using SDS-PAGE. During gel separation, the studied protein's anticipated size of roughly 24 kDa was seen. IFN 2b was successfully expressed, according to further evaluation by western blotting.

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