No abstract

Interferon alpha 2b gene (INF α2b) as a protein with antiviral and antitumor activities is potentially a valuable therapeutic proteins to work on. Prior to a large scale production of the target protein, it is recommended to examine it in an experimental scale, so that bacterial host could be a proper choice as it leads us to a deep insight of the subject. In this research, INF α2b sequence obtained from NCBI gene data bank, and after optimization it was subjected to be cloned and expressed in pET28a+. In order to primary examination of the target protein, Escherichia coli was considered as a prokaryotic expression system. IPTG induction of the protein in bacteria cells containing the construct pET: IFN, followed by resolving total proteins through SDS-PAGE. The expected size of the investigated protein, about 24kDa, observed through gel separation. Further assessment via western blotting confirmed successful expression of IFN α2b.

Concentrating on the interferon alpha 2b gene (INF 2b), a protein having antiviral and anticancer properties, could be beneficial therapeutically. It is advised to conduct an experimental examination of the target protein before having it produced on a big scale. The bacterial host is a good option since it allows us to gain a thorough understanding of the topic. The INF-2b sequence used in this study was obtained from the NCBI gene data library, and following optimization, it was cloned and expressed in pET28a+.Escherichia coli was chosen as the prokaryotic expression system for the target protein's initial analysis. Bacterial cells expressing the pET:IFN construct were IPTG-induced to produce the protein, which was then resolved using SDS-PAGE. During gel separation, the studied protein's anticipated size of roughly 24 kDa was seen. IFN 2b was successfully expressed, according to further evaluation by western blotting.

The effect of mobile microwave on antibiotic sensitivity of Staphylococcus ureus was estimated. One hundered samples of ear swab were collected from mobile phone users. They were subjected for S. aureus isolated and identification by Staph API-20 enzymes. Forty three of Staph. strains were selected as identical biochemical test.They were ordered into eight groups according to calling times of mobile users , ranged from zero (used as control) to three hundred and six hours of calling time.Seventeen different antibiotic discs sensitivity were measured for S. aureus.Microwave of mobile phone showed significant effect (P<0.01) on strains sensitivity according period of calling time. All strains showed more antibiotic resistance development at long calling period group than those have less calling (control and firsts groups). The present study may be explanation of mobile microwave of mobile effect on DNA expression as far as antibiotic resistance encoded by plasmid DNA.

New chalcones compounds were synthesis by reaction mefenamyl chloride with P-aminoacetophenone to from N–(4-acetyl phenyl)-2-(2/3- dimethyl phenyl) amino benzamide(A). The chalcones produced by Claisen – Schmidt condensation of compound A with substituted benzaldehydes [(2,4-dihydroxy(1), 3-NH2(2) and 4- OMe (3)] in ethanollic KOH solvent by using microwave irradiation. The new compounds were characterized by M.P, TLC, CHN, FT-IR, 1HNMR and 13CNMR. The biological screening data of the synthesized compounds were also presented. The theoretical study by using AM1 semi-empirical method emphasizes these compounds exists as trans configuration.