Humans are exposed to hepatocarcinogenic aflatoxins (Afs) through ingestion of contaminated foods as a result of poor storageful of susceptible grains or eating foods contaminated with aflatoxins animals and vegetables. This work aimed at evaluating the effect level of total (Afs) on human health. Study samples ninety persons divided into three groups (each /30 human). Group (1) no suffering liver disease as normal or negative control. Group (2) patients input (El Raghi hospital in Assuit) are suffering of liver inflammatory disease (HI). Group (3) patients input/or output (El Raghi hospital in Assuit) are suffering of hepatocellular carcinoma (HCC). Random samples were obtained from some foods (chicken egg; chicken, duck and rabbit liver) purchased from local market in Assuit. Total amount of aflatoxins was determined by using high-performance liquid chromatography (HPLC) instrumental. This study used 24 h recall and food frequency questioners for these groups to determination the type and amount of food which were intake. In serum humans measured total aflatoxins by used the enzyme-linked immunosorbent assay (ELISA) kits and ELISA technique. In addition oxidation tests are carried out for the studied individuals by using colorimetric kites to determine liver function (Alanine transaminase (ALT), Aspartate transaminase (AST), Total protein (TP),albumin (Alb), Bilirubin (Total, Direct), alkaline phosphatase (ALP), Glutathione peroxidase (GPX) and Malondialdehyde (MDA) by used spectroscopy instrument. Results indicated that were significant increase in liver function and biomarker antioxidants (GPX, MDA). Study concluded that these alterations in liver functions could be related to the development of liver damage in

Sulfuric acid manufacture generates huge quantity of sulfur sludge causing major environmental problems in their storage and disposal. On the other hand, molten sulfur should be filtered for sulfuric acid production to remove solids that may plug sulfur spray nozzles or accumulate on catalyst beds that increasing the pressure drop and decreasing operating durations. In this study, attempts were carried out using sulfur sludge wastes a filler in the manufacturing of filter aid. The filter aid using sulfur sludge is compared with the other filter aids such as Kessel Gohar diatomite (global product) and ABU ZAABLE 12 "AB12" (local product). Results showed that the prepared filter aid using sulfur sludge as filler decreases the ash content in filtrated sulfur than Kessel Gohar diatomite and AB12 with a ratio of about 26.4% and 39.4%, respectively, and thus results in increasing the operating durations due to decreasing the amount of ash deposited in the catalyst beds compared with filter aids of Kessel Gohar diatomite and AB12 with a ratio of about 25.2% and 39.4% respectively. On the other hand, sulfur sludge filter aid also improves filter operating duration than Kessel Gohar diatomite and AB12 with a ratio of about-1.2% and 32.4% respectively. Finally, sulfur sludge filler in manufacturing of filter aid can be used as a reusable material without any change on the ash content in filtrated sulfur and filter operating duration.