The aim of this study was to establish an experimental protocol for the artificial reproduction of royal carp ‘Cyprinus carpio’ with the hormonal induction of spawning. For this purpose, we conducted this study at an aquaculture farm in Khemis Meliana (Ain Defla, Algeria). The study included six royal carp broodstock, 4 females and 2 males. Hormone injection was performed in the dorsal muscle under the fin. The induction of carp was done successively in females by GnRH at a dose of 3 mg/kg and in males by hCG at a dose of 500 IU per kg weight. Fertilisation was done artificially by the dry method. After incubation of eggs, a binocular magnifying glass was used to check the condition of eggs and the development of the embryos over time. The results obtained show that the artificial reproduction of this species was possible, with success of ovulation and fertilisation, and hatching and growth of the larvae. Stripping was done after 24 h of hormonal stimulation at a temperature of 21°C. Disaggregation of eggs was performed efficiently with whole milk. The total number of eggs was 552,000 eggs with a weight of 1104 g. The average latency time was 22 hours. The hatching rate after 3 days of incubation on artificial spawning grounds at 21±1°C was 441,600 larvae (80%). Larvae food follow-up started from the 3rd day post-hatching with a combination natural food (rotifers) and artificial food. At the end of this experiment, we can conclude that it is possible to improve reproduction through the proper use of hormonal stimulation techniques and by improving feeding and abiotic factors that are dominant in fish farming.

The objective of this work was to set up an experimental protocol concerning artificial reproduction in the catfish Clarias gariepinus with the induction of spawning using GnRH. The experiment was carried out at an aquaculture farm in Khemis Meliana (Ain Defla), Algeria. In the study, five African catfish broodstock (3 females and 2 males) were used. Hormonal injection was made into the back muscle below the fin, and doses of GnRH were determined according to the weight of each individual. Fertilisation was performed artificially using the dry method. After incubating the eggs, a binocular magnifying glass was used to check egg condition and embryonic development over time. The results obtained show that artificial insemination of this species, and survival and growth of larvae, are possible. After injection with GnRH, C. gariepinus females displayed successful ovulation, fertilisation, larval hatching, and larval monitoring. For this species, a latency period of 22 hours was recorded, and approximately 35,700 larvae were obtained. The fertilization rate was 48%. At the end of this experiment, we can conclude that it is possible to improve reproduction through the proper use of hormonal stimulation techniques and by improving the diet and abiotic factors that are dominant in fish farming.