in silico modeling, using Psipred and ExPASy servers was employed to determine the structural elements of Bcr-Abl oncoprotein
(p210BCR-ABL) isoforms, b2a2 and b3a2, expressed in Chronic Myelogenous Leukemia (CML). Both these proteins are tyrosine
kinases having masses of 210-kDa and differing only by 25 amino acids coded by the b3 exonand an amino acidsubstitution
(Glu903Asp). The secondary structure elements of the two proteins show differences in five α-helices and nine β-strands which
relates to differences in the SH3, SH2, SH1 and DNA-binding domains. These differences can result in different roles played by the
two isoforms in mediating signal transduction during the course of CML.
The structure of human Methylenetetrahydrofolate Reductase (MTHFR) is not known either by NMR or by X-ray methods.
Phosphorylation seems to play an important role in the functioning of this flavoprotein. MTHFR catalyzes an irreversible reaction
in homocysteine metabolism. Phosphorylation decreases the activity of MTHFR by enhancing the sensitivity of the enzyme to SAdenosylmethione.
Two common polymorphisms in MTHFR, Ala222Val and Glu429Ala, can result in a number of vascular
diseases. Effects of the Glu429Ala polymorphism on the structure of human MTHFR remain undetermined due to limited
structural information. Hence, structural models of the MTHFR mutants were constructed using I-TASSER and assessed by
PROCHECK, DFIRE and Verify3D tools. A mechanism is further suggested for the decreased activity of the Ala222Val and
Glu429Ala mutants due to a decrease in number of serine phosphorylation sites using information gleaned from the molecular
models. This provides insights for the understanding of structure-function relationship for MTHFR.
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