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Biodiesel is an alternative source of fuel that is non-toxic and biodegradable than conventional fossil-based fuel. In this research, biodiesel was produced by the cultivation of Chlorella sorokiniana in Palm Oil Mill Effluent (POME). The lipid content of the algae was obtained using the solvent extraction method. Gas-chromatography mass spectrometer was used for the identification of Fatty Acid Methyl Esther (FAME). Based on the results of biodiesel analysis, the components of FAME confirmed in microalgae biomass are mostly saturated fatty acid and they include tridecyclic acid (C13:0), myristic acid (C14:0), pentadecylic acid (C15:0), palmitic acid (C16:0) and stearic acid (C18:0). These indicated that the biomass of C. sorokiniana after POME treatment has the potential to be used for bioenergy production.

Production of palm oil from palm oil mill industries has been facing a serious challenge of overcoming the oil loss as a result of the mechanical extraction process limitations, consequently, leading to economic and environmental impacts. Efforts were made to overcome this impasse by mechanical process, dissolve air flotation, solvent extraction and enzymatic treatment. Most of these methods applied are faced with a somewhat drawbacks. In this review, the various methods of residual palm oil recovery were discussed, while presenting a better method that could allow for optimum recovery with less or no environmental impact via microbial means in a solid state process. Biological process in a solid state condition with fungi such as the white rot basidiomycetes is considered the best for future application, due to the natural availability of the fungi and high degrading capability of lignocellulose biomass. This would significantly be of benefit in terms of abating the huge oil palm mesocarp fiber deposition and aid in meeting the environmental requirement of waste consumption from the source.

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