The conversion of waste obtained from agricultural processes into biocompatible materials (biomaterials) used in medical surgery is a strategy that will add more value in waste utilization. This strategy has successfully turned the rather untransformed wastes into high value products. Eggshell is an agricultural waste largely considered as useless and is discarded mostly because it contributes to pollution. This waste has potential for producing hydroxyapatite, a major component found in bone and teeth. Hydroxyapatite is an excellent material used in bone repair and tissue regeneration. The use of eggshell to generate hydroxyapatite will reduce the pollution effect of the waste and the subsequent conversion of the waste into a highly valuable product. In this paper, we reviewed the utilization of this agricultural waste (eggshell) in producing hydroxyapatite. The process of transforming eggshell into hydroxyapatite and nanohydroxyapatite is an environmentally friendly process. Eggshell based hydroxyapatite and nanohydroxyapatite stand as good chance of reducing the cost of treatment in bone repair or replacement with little impact on the environment.

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Wastewater is untreated water that has high amount of nutrients such as nitrate, phosphate, ammonium and chemical oxygen demand (COD). When it is discharged into watercourse, it affects human and aquatic biota. The application of photosynthetic bacteria is considered bio-friendly system than the conventional one. Hence, the present study investigates the effectiveness of robust strain of photosynthetic bacteria for nitrate removal under different concentrations of 85, 135, 190, 235 and 320 mg/L. Serial dilution techniques was used for the isolation of the bacteria. The results showed that three bacterial isolate were obtained and were both screened for nitrate reduction ability. The isolate was able to remove 91, 90, 71, 67 and 55% of nitrate at 85, 135, 190, 235 and 320 mg/L respectively. The bacteriochlorophyll of the isolate was detected at peak range between 689-710 nm. The morphological, physiological and biochemical characterization showed that the isolate was identified as Rhodopseudomonas sp. The nutrient removal yield of the nitrate under different concentrations was found to be at range of 0.01- 0.033 g-1 DCW g-1 NO3.- This study suggested that the strain could be used as an efficient bacterial candidate for the treatment of wastewater containing high amount of nitrate.

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