No abstract

Melamine and its metabolites pose health concern as they are used in various industrial products including feed and drugs. There are a limited number of studies on melamine and cyanuric acid cytotoxicity and cellular damage without a certain conclusion. The present study aimed to evaluate melamine, cyanuric acid and its combined cytotoxic effects using 3-(4.5dimethylthiazol-2-yl) methyl thiazole tetrazolium (MTT) bromide test. The study also evaluated apoptotic and necrotic effect using a double staining method of Hoechst 33342 and propidium iodide. Melamine, cyanuric acid and their combination (1:1) were applied to L929 fibroblasts and Chinese hamster ovary (CHO) cells at various concentrations (1000 µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL and 62.5 µg/mL). At the highest concentration (1000 µg/mL), the cell viability dropped down approximately to 50% both in CHO cells and L929 cells. Melamine, cyanuric acid and their mixture caused cytotoxicity in CHO cells and L929 fibroblasts in dose-dependent manner. Cell death occurred through both apoptosis and mainly necrosis. Both cell types were more sensitive to the mixture of melamine and cyanuric acid and, furthermore, CHO cells were more sensitive than L929 fibroblasts. As a result, melamine, cyanuric acid and their combination caused cytotoxicity in CHO cells and L929 fibroblasts. Further studies should be conducted in different cell lines. These studies should also aim to reveal the mechanism of cytotoxicity and related pathways.

Our study demonstrated that the mRNA levels of the genetic markers considered to play a role in adipogenesis were different in short- and long-term obesity models formed in C57BL/6J mice using HFD.

Araştırmacılar, ileri teknolojiye sahip farklı hastalıklar için farklı temeller bildirmişlerdir. Bu arada, son zamanlarda temel hücresel mekanizmaların etkileşimlerini netleştirmek için birçok araştırma yapılmıştır. Bu nedenle, hücre iskeleti araştırmaları bu eğilim nedeniyle önem kazanmaktadır. Hücre iskeleti yapıları, hücre çekirdeği, sitoplazma ve ayrıca hücre dışı matriks arasındaki bağlantılardan sorumludur, böylece hücresel moleküller ve sinyalizasyon iletimi arasında bir iletişim bağlantısı oluşturur. Hücre iskeleti, üç çeşit protein filamanından oluşur: aktin filamentleri, ara filamentler ve mikrotübüller. Bu makalede, bu yapıların niteliği kısaca özetlenmiştir ve hücre iskeleti bileşenleri ile yaygın hastalıklar arasındaki ilişkiyi bildiren literatür taraması sunulmuştur.