Heart failure (HF) incidence could cause further complications to other body organs, which might sometimes be fatal, and is accompanied by various biochemical alterations i.e. enzymatic changes. The objective of this study was to measure the activity of gamma glutamyl transferase (GGT) as an early diagnostic indicator for HF patients; and to isolate the iso-enzymes for the purpose of finding the Michaelis-Menten constant (Km) and the maximum velocity Vmax of each iso-enzymes which enable follow up the development of HF disease. Samples of blood sera were collected from 120 patients of both genders (70 males and 50 female, aged 30 -38 years old). Partial purification of iso-enzyme GGT was performed by precipitation, gel filtration, and ion exchange of the two iso-enzyme (I and II). The purity of the enzyme was confirmed by using Sodium dodecyl sulfate-polyacryl amide gel electrophoresis (SDS-PAGE) into two clear bands. The results were compared with other 80 samples of healthy volunteers whose ages ranged between 25 -78 years old, used as control. There has been a significant increase (p ≤ 0.01) in the activity of the enzyme GGT in the heart failure patient (66.9 ± 1.7 IU/L) in comparison with control (12.07 ± 0.60 IU/L). It is concluded that measurements of the iso-enzyme GGT could well benefit as a clear indicator criteria in prognosis of heart failure.

For thousands of years plants have been an essential medicinal source with qualities. Empty fruit bunches (EFB) have medicinal values. Ten grams of powdered sample was extracted with 50 mL ethanol overnight and filtered through ash less filter paper, this plant’s ethanol extract has been analyzed using Gas Chromatography–Mass Spectrometry (GC-MS), while the compound mass spectra contained in the extract has been matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis provided different peaks determining the presence of eight different phytochemical compounds namely10-Undecenoic acid, Propanoic acid, Cyclopentane acetic acid hydrazide,1,4-Cyclohexanedicarbonitrile.The compounds were identified by comparing their retention time and peak area with literature and by interpreting the mass spectra. Many of them have antioxidant, anti-inflammatory, antimicrobial, antifungal, antitumor, antiviral, anticonvulsant, analgesic and antidiabetic properties.