Ganoderma was isolated from an apple tree in the Tikrit region (Iraq), according to phenotypic characters and phylogenetic analyses based on internal transcribed spacer ITS 1 - 5.8S - ITS4 rDNA sequences, this isolate identified as G. resinaceum and registered with accession number MN448375 in the NCBI Database. We described a novel media for artificial cultivation of G. resinaceum, composed of wheat straw as basic substrate amended with premix (paper wastes, poplar sawdust and rice hulls at 1:1:1 (W:W:W) the most suitable medium was wheat straw amended with 20 % of premix, in this medium the highest yield (biological efficiency) of  G. resinaceum basidiocarps was recorded. The cultural G. resinaceum characteristics grown in all tested media were also describted. HIGHLIGHTS Isolation and identification of the fungus Ganoderma resinaceum was very important for the purpose of adding this fungus to the mycoflora The production of the fruit bodies of Ganoderma resinaceum is one of the important economic factors, as these fruits are the raw source for the production of many medicinal materials The components of the nutrient medium that are uniquely mentioned in this study are the main key to the growth of this mushroom and the production of its fruiting bodies The production of mushroom Ganoderma resinaceum using agricultural waste, such as wheat straw and paper waste, contributes to the environmental cleaning of these wastes and enters the recycling of resources to produce useful products GRAPHICAL ABSTRACT