Paracetamol (Acetaminophen) was used for long time in poultry as an antipyretic drug and as a growth stimulator. However, high doses of paracetamol cause unpleasant side effects such as hepatorenal toxicity as mirrored by depletion of glutathione reserve, increase lipid peroxidation and increase liver enzymes or even sudden death with lethal doses. Therefore, this study was designated to evaluate the ameliorative effect of L. methionine, L. carnitine, choline and/or silymarin on the hepatotoxic effect induced by high doses of paracetamol. The study was applied on 80 chicks (from 1 till 33 days old) in special cages divided into 8 groups each one contained 10 chicks. The 1 st group was used as a control, while the next 2-5 groups were supplemented with L. methionine, L. carnitine, choline and silymarin, respectively with doses as recommended by NRC, the 5 th group was supplemented with silymarin (1 g/kg diet), the 6 th group was supplemented with a mixture of the 4 supplements, the 7 th group (hepatic intoxicated group) was given paracetamol (650 mg/kg diet for 7 days), and the 8 th group was administrated paracetamol with a mixture of the 4 supplements. Serum samples were collected to determine levels of lipid profile [triglycerides (TG) and total cholesterol (TC)], liver damage enzymes [alanine transaminase (ALT), aspartate transferase (AST)], lipid peroxidation marker malondialdehyde (MDA), and activity of antioxidant enzymes [glutathione reductase (GR), superoxide dismutase (SOD)]. The obtained results revealed that there was a significant improvement in all measured serum biochemical parameters and final body weight gain in the combined group (4 supplements + paracetamol) as compared to the paracetamol group. These data conclude that supplementation of poultry diets with L. methionine, L. carnitine, choline and silymarin can improve the negative effect of paracetamol through increasing the body weight gain and antioxidant activity of glutathione and superoxide dismutase and decreasing malondialdehyde, liver enzymes, cholesterol and triglycerides.