Basil and binahong leaf are plants that can be used as medicine. Basil leaf contain alkaloids, flavonoids, saponins and tannins. Binahong leaf contain alkaloids, flavonoid and tannins. These compounds have the ability as an antioxidant to inhibit free radicals. The purpose of this study was to determine the antioxidant activity of a combination of basil leaf extract and binahong leaf. The method used in testing the antioxidant activity is the DPPH free radical scavenging method (1,1-diphenyl-2-pikrihidrazil). Simplicia powder of basil leaf and binahong leaf were extracted by maceration using 70% ethanol solvent. Each extract and extract combination was tested for antioxidant activity against DPPH by measuring the absorbance of DPPH at a wavelength of 516.5 nm. The results showed that the IC50 value for ethanol extract of basil leaf was 42.33 ppm, ethanol extract of binahong leaf was 51.57 ppm, and the IC50 value from each of the combination of ethanol extract of basil leaf and binahong leaf at a ratio of 1:1; 1:2 ; 2;1 was 59.71 ppm ; 77.18 ppm ; 24.33 ppm. Based on the results of the study it can be concluded that the antioxidant activity of the combination of ethanol extract of basil and binahong leaf is very strong compared to the singular both. The combination of ethanol extract of basil leaf and binahong leaf in a ratio of 2:1 has very strong antioxidant activity.
Hiperlipidemia merupakan suatu keadaan meningkatnya kadar lipid darah yang ditandai dengan meningkatnya kadar kolesterol total, Low Density Lipoprotein (LDL), dan trigliserida dalam darah yang melebihi batas normal. Sampai saat ini telah banyak obat yang digunakan untuk penanganan hiperlipidemia baik obat sintetik dan obat herbal. Salah satu obat herbal yang telah digunakan oleh masyarakat Indonesia adalah buncis (Phaseolus vulgaris L.). Tujuan penelitian ini adalah untuk mengetahui pengaruh beberapa ekstrak (n-heksan, etilasetat dan air) buah buncis terhadap penurunan kadar kolesterol tikus jantan galur Sprague Dawley yang telah diinduksi kuning telur puyuh, PTU 0,02% dan asupan pakan aterogenik selama 14 hari. Pengukuran kadar kolesterol dilakukan secara enzimatis dengan alat kolesterolmeter Easy Touch®. Hasil fraksinasi ekstrak etanol buncis dengan pelarut n-heksan, etil asetat, dan air diujikan selama 7 hari dengan dosis 50 mg/kg BB diperoleh fraksi air sebagai fraksi yang paling aktif menurunkan kadar kolesterol darah. Pada uji lanjutan fraksi air dilakukan terhadap 5 kelompok perlakuan (5x5 ekor), yaitu kelompok 1 (Na CMC 0,5%) sebagai kontrol negatif, kelompok 2 (simvastatin 0,18 mg/200 g BB) sebagai kontrol positif dan variasi dosis fraksi air 50 mg/kg BB, 100 mg/kg BB serta 150 mg/kg BB (kelompok 3, 4, dan 5). Selama 21 hari perlakuan tikus tetap diberikan pakan aterogenik dan pengukuran kadar kolesterol dilakukan pada hari ke 0, 14, 21, 28, dan 35. Hasil uji lanjutan menunjukkan bahwa fraksi air ekstrak buncis pada dosis 50 dan 100 mg/kg BB tidak memiliki perbedaan yang nyata dengan simvastatin, sedangkan pada dosis 150 mg/kg BB menunjukkan perbedaan yang nyata dangan simvastatin dalam menurunkan kadar kolesterol.
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