DDT and metabolites, dieldrin, heptachlor epoxide, and lindane pesticide residues accumulated in the body tissues and the eggs of laying hens in direct proportion to the concentration of the pesticide or its parent compound in feeds. Methoxychlor residues did not accumulate at any of the concentration levels used in the study. Plateaus of residue accumulation occurred independently for each pesticide concentration after 6 to 12 weeks of feeding at which time, as long as the concentration in the feed remained constant, the apparent elimination or metabolism of the pesticide residue balanced the intake. The concentration of residues in eggs at the plateau was directly proportional to and dependent on the concentration in the feed. The same direct relationship existed between the concentration of pesticide residue in the feed and the residue in the body tissues. The significance of pesticide contamination in eggs resulting from feeding levels of 0.1 p.p.m. and less is highly questionable because concentrations in eggs were generally below 10 p.p.b. and very seldom exceeded 50 p.p.b. Although higher concentrations of pesticide residues were found in the body fat at the 0.1 p.p.m. level, the transfer of residue to the egg did not appear to be important. ABSTRACTThe effect of three lighting regimes on broiler performance was determined. An environmentally controlled poultry house with 12 light controlled chambers was utilized and chicks received A) continuous illumination, B) illumination for 12 hours followed by 15 minutes of illumination each 2 hours or C) 15 minutes illumination each 2 hours. Two trials

General and broad occurrences of minute organochlorine pesticide residues were indicated in feedstuffs used in poultry feeds in the United States. Corn, soybean meal, alfalfa meal, fish meal, and fat samples were collected and submitted bimonthly over a 24 month period from Maine, Georgia, Texas, Ohio, and California and analyzed for aldrin, dieldrin, heptachlor, heptachlor epoxide, lindane, DDE, DDD, o,p-DDT, p,p'-DDT, and methoxychlor. Concentration of individual pesticides when occurring were generally in the range of 10 to 50 p.p.b. although higher concentrations occasionally occurred. DDT and its metabolites were the most frequently occurring organochlorine pesticides detected. The highest concentration and frequency of residues detected were associated with fish meal and fat samples. Occurrence of residues in corn and soybean meal was generally infrequent and at very low concentrations. The presence of pesticide residues was very closely correlated with the areas of high crop production and hence greater pesticide use. In general it appears that poultry feeds formulated from feedstuff sources as analyzed in this study (corn and soybean meal constituting by far the major part of the poultry ration) would not contain organochlorine pesticide residues large enough to cause any major contamination problems with poultry products.

Heptachlor and heptachlor epoxide residues in trace amounts occurred in alfalfa harvested from fields treated the previous fall at 0.25, 0.50, and 1.00 pound of heptachlor per acre.Heptachlor epoxide residues subsequently appeared in the fat tissue and milk of cows that ingested the treated hay over a period of 30 days. The highest concentration of residue in milk occurred between the 18th and 24th days of the feeding period and averaged 13, 26, and 49 p.p.b. for the test animals in each group. Re-

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