Background and Objective: Unproductive soil has been a major problem to farmers, recent interest in the use of a stable organic manure (biochar) as soil amendment and poultry manure to increase soil fertility is also increasing. Hence, the experiment was conducted to assess the condition of poultry manure and biochar on the growth and yield of tomato. Materials and Methods: A greenhouse experiment was conducted at the Federal College of Forestry Ibadan, using polyethylene bag with 5 kg soil collected at two (2) soils depths, 0-15 cm and 15-30 cm depth at the botanical garden, Federal College of Forestry Ibadan. The experiment was arranged in a completely randomized design (CRD) with four replicates. The treatments used were poultry manure (5t/ha), biochar (10t/ha), mixture of poultry manure and biochar (1:1) and control (no amendment).The tomato seeds (UC82B) were sourced from NIHORT, Ibadan and raised in a germination basket. Data were collected weekly on plant height, collar diameter and number of leaves, 50% days to flowering and yield of tomato plants from two weeks after transplanting (WAT). Data collected were analyzed statistically using ANOVA while means were separated using LSD (p<0.05) where applicable. Results: In 0-15 cm soil, the growth variables (plant height, number of leaves and collar diameter) increased with various treatments used. The highest tomato height was observed with the application of biochar (10t/ha). All the treatments applied affected the number of tomato leaves at all weeks after transplanting (WAT) with poultry manure 5t/ha having the highest number of leaves at 7WAT, while collar diameter was significantly affected by various treatments from 2 to 5 WAT, with the mixture of P.M + BIO (1:1) recording the highest value and the control plot recorded the least values in all weeks. However, in 15-30 cm soil, there was  significant difference in the number of leaves of tomato among all the amended soil from 5 to 7 WAT, the highest tomato leaves were obtained when the soil was amended with poultry manure (5t/ha) and can be compared with other treatments. Interaction of poultry manure and biochar(1:1) and poultry manure 5 t/ha resulted in highest height of tomato plant from 3-7 WAT as compared to the control that had the least value but were not significantly different from the sole application of  biochar 10t/ha. Interaction of poultry manure + biochar (1:1) and poultry manure (5t/ha) gave the highest tomato flowering and fruits. Conclusion: Based on this result, biochar and poultry manure when added to soil, boosted the mineralization of the soil after amendment.