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O gênero Scopulariopsis spp. engloba fungos cosmopolitas que normalmente são encontrados na poeira doméstica ou no solo. Raramente causam infecção em humanos, todavia podem ocasionar onicomicose, otomicose e panoftalmite, se introduzido nos olhos devido a feridas penetrantes. Em determinadas situações podem resultar em micoses cutâneas, profundas e potencialmente infecções sistêmicas, estas normalmente associadas a pacientes imunodeprimidos (COX; IRVING, 1993). Relatar a ocorrência de Scopulariopsis spp. proveniente de swab lesional de um felino (Felis catus domesticus) em tratamento para esporotricose na cidade de Guaíra, estado do Paraná, Brasil. Um felino macho, não-castrado, escore corporal de 2,5/5 e dois anos de idade, foi submetido à colheita de material lesional para isolamento de colônia de Sporothrix spp. O mesmo residia em uma casa com outros 50 gatos, quatro galináceos, três cães e possuía acesso à rua, contudo há uma semana se encontrava isolado juntamente com outros quatro felinos, também com esporotricose, em um cômodo dentro do ambiente interno da casa da tutora que também estava em tratamento para a doença. O animal apresentava lesões ulcerativas em ponta de orelha, na região interescapular, região do metacarpo esquerdo, face e testículo e se encontrava em tratamento utilizando itraconazol 100mg, iodeto de potássio 30mg e silimarina 100mg. Procedeu-se a colheita do material com swab nasal, swab de unhas e swab lesional, todas as amostras foram armazenadas em solução salina 0,9% estéril e refrigerados, até o momento do processamento. O material foi estriado em placa contendo ágar Sabouraud com gentamicina, e submetido a crescimento em temperatura ambiente. Após sete dias, verificou-se o crescimento de uma colônia fúngica de coloração brancacenta, irregular e algodonosa no estriado proveniente de swab lesional. Procedeu-se então o teste da fita adesiva corada com lactophenol cotton blue e analisada por meio de microscopia óptica. Na microscopia foi possível verificar hifas septadas, conidióforos com anéis hialinos, conídios piriformes com base truncada, de parede lisa em corrente, sendo característico do gênero Scopulariopsis spp. Esses dados demonstram que mais estudos referentes aos mecanismos de patogenicidade deste fungo devem ser realizados, visto que a presença deste no local da lesão causada pela multiplicação do Sporothrix spp. pode estar relacionada ao desenvolvimento de micoses cutâneas, o que pode favorecer o aumento das lesões ocasionadas pelo Sporothrix, levando ao questionamento sobre a eficácia do tratamento no paciente felino.

This work aimed to perform the molecular identification of fungi mango fruits, in addition to verifying the effect of the essential oils for microbial control. Pericarp segments containing injured and healthy areas of mango fruits were initially disinfested, and then incubated in PDA culture medium at 25 °C. From all isolated material, UDC03, UDC04, UDC09 and UDC12 were preselected, which presented homogeneous cultures. The UDC04 and UDC12 isolates were submitted to the sequencing of the ITS region for presenting mycelial growth both greater and morphologically distinct of fungi from Colletotrichum genus. When BLAST is used in species identification, it was observed that UDC04 and UDC12 belong to Botryosphaeria dothidea and Neofusicoccum parvum, respectively. Regarding the control of UDC04, it was observed that BDA culture medium containing 1.5 to 2% of lemongrass and eucalyptus oils resulted in complete inhibition. For UDC12, mycelial growth inhibition was detected with the use of 1% and 1.5% essential oils of eucalyptus and lemongrass, respectively. The chia essential oil was not effective for the isolates control. Finally, the results reveal the importance of molecular identification of these phytopathogens associated with the establishment of an alternative control of fruits due to problems of contamination with chemical products.

The objective of this study is to compare the direct fecal smear (DFS) and centrifugal sedimentation (CS) methods in the detection of Cryptosporidium spp. oocysts in fecal samples of dairy calves. One hundred and fourteen fecal samples were collected from calves aged up to six months from 10 dairy farms located in Palotina and Francisco Alves, Paraná, Brazil. The microscopic analysis revealed the presence of Cryptosporidium spp. oocysts in 51.75% (59/114) of the samples in both methods. In CS, 48.25% (55/114) of the samples were positive, while in DFS slides, only 6.14% (7/114) were positive. Only 4 samples were positive exclusively in DFS. To ensure that there were no false-negative results in the microscopic analysis, the 55 samples that were negative in both DFS and CS were selected for molecular analysis using the nested PCR (nPCR). Of these 55 samples, 24% (13/55) were positive and forwarded for sequencing part of the genome, which made it possible to identify C. parvum, C. bovis and C. ryanae. Besides the characterization of the Cryptosporidium species, it was possible to identify bacteria of the genus Acinetobacter interfering directly in the analyzed samples. The microscopic analysis also revealed higher sensitivity when CS was used to make the fecal smears. However, some samples that were negative in this technique had positive PCR results. Thus, molecular analysis is indicated to confirm cases of Cryptosporidium spp. Further studies are necessary to prove the specificities of the used primers since the results obtained in nPCR were positive for the protozoan but, when genetic sequencing was performed, Acinetobacter spp. was identified.