The aim of this work was to develop an analytical method for determining acrylamide in potato-based products by gas chromatography and tandem mass spectrometry analysis (GC-MS/MS) using solid-phase extraction (SPE) clean-up. Different conditions for extraction and clean-up of AA extracts were studied on a potato crisps reference material (RM) with the value of 625 ± 45 μg/kg: the solvent volume used for AA elution, the extraction water temperature, the volume of hexane used, and also the addition of Carrez solutions. The SPE cartridge sorbents contain silica-based C-18 groups (anion and cation exchangers) and polymers (polystyrene-divinylbenzene). After SPE clean-up, extracts were derivatized with bromine compounds. A good efficiency for AA extraction and a cleaned-up extract from this matrix were obtained when the SPE procedure was carried out with water at room temperature, simultaneous with hexane, without Carrez solutions, by using two types of SPE cartridges (Isolute Multimode; Isolute ENV+), and 5 mL of elution solvent. The SPE clean-up procedure functionality was demonstrated by the results obtained in the Food Analysis Performance Assessment Scheme proficiency test (z-score: −0.8) on French fries (precooked) matrix and also by comparative analysis with a laboratory procedure, validated and accredited on cereal-based food matrices, in which liquid–liquid extraction and clean-up through a florisil column were realized (RSD(R) = 2.23–5.10%).

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The chemical group comprising polycyclic aromatic hydrocarbons (PAHs) has received prolonged evaluation and scrutiny in the past several decades. PAHs are ubiquitous carcinogenic pollutants and pose a significant threat to human health through their environmental prevalence and distribution. Regardless of their origin, natural or anthropogenic, PAHs generally stem from the incomplete combustion of organic materials. Dietary intake, one of the main routes of human exposure to PAHs, is modulated by pre-existing food contamination (air, water, soil) and their formation and accumulation during food processing. To this end, processing techniques and cooking options entailing thermal treatment carry additional weight in determining the PAH levels in the final product. With the background provided, this study aims to provide an improved understanding of PAH occurrence in meat, edible oils, and cereal products. The factors influencing PAH formation, including operational conditions and parameters, product composition, and storage settings, are described. The discussion also addresses reduction directions with respect to influencing factors informing the choice of the employed technique, fuel type, time–temperature settings, and ingredient variations. Considering the disparities caused by wide variations in PAH contamination, challenges associated with PAH control requirements are also outlined in the context of relevant preventive approaches during food processing.

For simultaneous separation, detection, identification and quantification of fatty acid methyl esters from oils/fats, a GC-MS method was developed and validated. The method is based on fat extraction and transesterification of fatty acids to fatty acid methyl esters. Samples of sunflower oil, refined non-hydrogenated palm oil, fish oil and lard, demonstrated the applicability of the proposed method. Fatty acid methyl esters determination and quantification was realized by using internal standards, and applying relative response factors, and without using internal standards by applying correction factors. Linearity, sensitivity, precision, accuracy, recovery and robustness were determined. The method is sensitive enough to simultaneously quantify 26 compounds, when using internal standard (absolute concentration), and 40 fatty acids without using internal standard (relative concentration). Accuracy was achieved by using a reference material, peanut butter (SRM ® 2387). The results have shown that the proposed method could be considered an effective tool for analyzing the fatty acid profile of food.

The aim of this study was to investigate the influence of some pre-treatment applications toward acrylamide mitigation in potatoes fried in domestic conditions modeled after those found in Romania, by using a pan and a fryer. Before being fried in a pan, potato strips were treated in one of the following ways: soaked in cold water for 15, 60, and 120 min (a); soaked in hot water at different combinations of temperatures and durations (60, 70, 80 °C for 5, 10, 15 min) (b); soaked in a NaCl solution (c), and; in a citric acid solution (d) both solutions of 0.05% and 1% concentration for 30 min. For potatoes fried in a fryer, the (a) pre-treatment and soaking in water at 80 °C for 5, 10, and 15 min were applied. Untreated samples were used as a control. French fries were analyzed in terms of moisture and acrylamide content, color, and texture parameters. The pre-treatments applied reduced the acrylamide content in French fries by 4–97% when fried in the pan and by 25–47% when fried in the fryer. Acrylamide content of French fries was negatively correlated with L* parameter and moisture content and positively correlated with a* parameter. The pre-treatments applied can be used successfully by consumers to reduce acrylamide content.